Otro
Penicillinase-based amperometric biosensor for penicillin G
Registro en:
Electrochemistry Communications. New York: Elsevier Science Inc, v. 38, p. 131-133, 2014.
1388-2481
10.1016/j.elecom.2013.11.022
WOS:000331504300035
Autor
Goncalves, Luis Moreira
Callera, Welder F. A.
Sotomayor, Maria Del Pilar Taboada
Bueno, Paulo Roberto
Resumen
A biosensor for penicillin G was created by immobilizing penicillinase to a gold electrode by means of a cysteine self-assembled monolayer. The biosensor amperometrically monitored the catalytic hydrolysis of penicillin in a very sensible manner. Furthermore, it was successfully used to measure the Michaelis-Menten enzymatic constant and a low limit of detection of 4.5 nM was obtained. (C) 2013 Elsevier B.V. All rights reserved. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)