| dc.creator | Del Arco, Jon | |
| dc.creator | Sánchez Murcia, Pedro Alejandro | |
| dc.creator | Mancheño José Miguel, José Miguel | |
| dc.creator | Gago, Federico | |
| dc.creator | Fernandez Lucas, Jesus | |
| dc.date | 2018-11-16T01:03:25Z | |
| dc.date | 2018-11-16T01:03:25Z | |
| dc.date | 2018-05-23 | |
| dc.date.accessioned | 2023-10-03T20:06:46Z | |
| dc.date.available | 2023-10-03T20:06:46Z | |
| dc.identifier | 0175-7598 | |
| dc.identifier | http://hdl.handle.net/11323/1093 | |
| dc.identifier | Corporación Universidad de la Costa | |
| dc.identifier | REDICUC - Repositorio CUC | |
| dc.identifier | https://repositorio.cuc.edu.co/ | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/9174284 | |
| dc.description | In our search for thermophilic and acid-tolerant nucleoside 2′-deoxyribosyltransferases (NDTs), we found a good candidate in an enzyme encoded by Chroococcidiopsis thermalis PCC 7203 (CtNDT). Biophysical and biochemical characterization revealed CtNDT as a homotetramer endowed with good activity and stability at both high temperatures (50–100 °C) and a wide range of pH values (from 3 to 7). CtNDT recognizes purine bases and their corresponding 2′-deoxynucleosides but is also proficient using cytosine and 2′-deoxycytidine as substrates. These unusual features preclude the strict classification of CtNDT as either a type I or a type II NDT and further suggest that this simple subdivision may need to be updated in the future. Our findings also hint at a possible link between oligomeric state and NDT’s substrate specificity. Interestingly from a practical perspective, CtNDT displays high activity (80–100%) in the presence of several water-miscible co-solvents in a proportion of up to 20% and was successfully employed in the enzymatic production of several therapeutic nucleosides such as didanosine, vidarabine, and cytarabine. | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | Applied Microbiology And Biotechnology | |
| dc.rights | Atribución – No comercial – Compartir igual | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.rights | http://purl.org/coar/access_right/c_abf2 | |
| dc.subject | Enzymatic Synthesis | |
| dc.subject | Extremophiles | |
| dc.subject | Homology Modeling | |
| dc.subject | Nucleoside 2′-Deoxyribosyltransferase | |
| dc.subject | Nucleoside Analogues | |
| dc.title | Characterization Of An Atypical, Thermostable, Organic Solvent- And Acid-Tolerant 2′-Deoxyribosyltransferase From Chroococcidiopsis Thermalis | |
| dc.type | Artículo de revista | |
| dc.type | http://purl.org/coar/resource_type/c_6501 | |
| dc.type | Text | |
| dc.type | info:eu-repo/semantics/article | |
| dc.type | info:eu-repo/semantics/publishedVersion | |
| dc.type | http://purl.org/redcol/resource_type/ART | |
| dc.type | info:eu-repo/semantics/acceptedVersion | |
| dc.type | http://purl.org/coar/version/c_ab4af688f83e57aa | |
