Artigo
Antioxidant activity and duodenum transporter gene expression in quail fed citric acid
Registro en:
2221-4062
Creative Commons Attribution 4.0 South African License
Autor
Santana, Thaís Pacheco
Jesus, José Aparecido Santos de
Bastos, Marisa Silva
Nunes, Adriano Silva
Oliveira Júnior, Gregório Murilo de
Nascimento, Carlos Souza do
Barbosa, Leandro Teixeira
Brito, Claudson Oliveira
Gasparino, Eliane
Del Vesco, Ana Paula
Institución
Resumen
The study aimed to evaluate the effects of citric acid supplementation on the performance and the
gene expression in the duodenum of Japanese quail. Genes related to antioxidant activity: superoxide
dismutase (SOD) and glutathione peroxidase 7 (GPX7); and genes related to nutrient uptake in the
enterocytes’ apical membrane: sodium-glucose cotransporter 1 (SGLT1, responsible for the glucose
absorption), glucose transporter 2 (GLUT2) and B0
AT1 (responsible for the absorption of neutral amino acids
in brush board membrane) were evaluated. For this, 270 laying quails (Coturnix japonica) were fed a diet
supplemented with 0%, 0.6% or 1.2% of citric acid. Gene expression was evaluated by the quantitative
reverse transcription-polymerase chain reaction (qRT-PCR) at 35 days old (n = 5). The highest expression of
the SGLT1 gene was observed in quails fed the 1.2% citric acid supplemented diet (0.120 AU). Birds fed
diets with both levels of citric acid supplementation showed a higher B0
AT1 gene expression than quails fed
no citric acid. Quails fed the 1.2% of citric acid supplemented diet had the lowest SOD gene expression
(0.3455 AU). The birds receiving the diets supplemented with citric acid showed a lower GPX7 gene
expression than the quails fed the 0% citric acid diet. These results suggests that the effect of citric acid on
gene expression in the digestive tract may have contributed to the greater weight gain and lower feed intake
observed in birds fed diets supplemented with citric acid.