Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

Leal, C. A. G.; Carvalho-Castro, G. A.; Cottorello, A. C.; Leite, R. C.; Figueiredo, H. C. P.

Artigo

Registro en:

LEAL, C. A. G. et al. Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD. Brazilian Journal of Microbiology, São Paulo, v. 44, n. 3, July/Sept. 2013.

http://repositorio.ufla.br/jspui/handle/1/57882

https://repositorioslatinoamericanos.uchile.cl/handle/2250/9044195

Autor

Leal, C. A. G.

Carvalho-Castro, G. A.

Cottorello, A. C.

Leite, R. C.

Figueiredo, H. C. P.

Institución

Universidade Federal de Lavras (Brasil)

Resumen

The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.

Materias

Clinical samples

Sensitivity

White spot syndrome virus (WSSV)

Real-time PCR

White spot disease (WSD)

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