Artigo
Capture of lysozyme on macroporous cryogels by hydrophobic affinity chromatography
Registro en:
Autor
Oliveira, Ana Cristina Freitas de
Neves, Isabelle Cistina Oliveira
Saraiva, Jamile Alves Meireles
Carvalho, Marcelle Flores Fernandes de
Batista, Gioanni Aleixo
Veríssimo, Lizzy Ayra Alcântara
Resende, Jaime Vilela de
Institución
Resumen
Different protocols for functionalization of cryogels with L-tryptophan were tested for the lysozyme adsorption. The effect of ionic strength and pH on the adsorptive capacity (q) was investigated. The morphological, thermal, and mechanical properties of the cryogels were determined. A maximum adsorption for lysozyme (58.030 mg g–1) was achieved when 5% (v/v) of glutaraldehyde (at pH 6.0) and no methanol were used during the cryogel functionalization. The hydrophobic cryogel presented a porosity of 0.666. A strong single band at 14.3 kDa was visualized in SDS-PAGE, indicating that the hydrophobic affinity cryogels was efficient for the lysozyme capture.