Artigo de Periódico
Avaliação de seis testes sorológicos no diagnóstico da brucelose bubalina
Registro en:
1678-5150
0100-736X
Autor
MOLNÁR, László
MOLNÁR, Lászlóné Éva
NUNES, Emilia do Socorro Conceição de Lima
DIAS, Hilma Lúcia Tavares
Institución
Resumen
Four hundred and forty buffalo sera, selected from about 1,200 blood samples of another study, were examined. The samples were tested by six serological methods: two of agglutination, two of indirect ELISA and two of competitive ELISA. To determine the relative sensitivity and specificity of different tests, animals with a positive result to competitive ELISA of the FAO/IAEA were considered as infected. The relative sensitivity of competitive ELISA, indirect ELISA with conjugate anti-bovine light chain monoclonal antibody labelled with HRPO, indirect ELISA with anti-bovine IgG conjugate, rose Bengal test and rapid slide agglutination test was 100%, 98.57%, 97.14%, 91.42% and 79.28%, and the relative specificity 99.33%, 97.33%, 95.66%, 94.00% and 86.33%, respectively. The value of the different serological tests for the diagnosis of brucellosis is discussed.