| dc.creator | YOSHIMURA, TANIA M. | |
| dc.creator | SAUTER, ISMAEL P. | |
| dc.creator | RIBEIRO, MARTHA S. | |
| dc.date | 2021 | |
| dc.date | 2022-03-29T18:31:39Z | |
| dc.date | 2022-03-29T18:31:39Z | |
| dc.date.accessioned | 2023-09-28T14:21:44Z | |
| dc.date.available | 2023-09-28T14:21:44Z | |
| dc.identifier | 1867-2450 | |
| dc.identifier | http://repositorio.ipen.br/handle/123456789/32895 | |
| dc.identifier | 6 | |
| dc.identifier | 13 | |
| dc.identifier | 10.1007/s12551-021-00844-3 | |
| dc.identifier | 0000-0002-4203-1134 | |
| dc.identifier | Sem Percentil | |
| dc.identifier | 90.00 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/9003114 | |
| dc.description | INTRODUCTION
Photobiomodulation (PBM) has been gaining importance in
a wide range of medical fields in the past few years, particularly
in stem cell-based regenerative medicine. Improving
in vitro cell proliferation, differentiation and viability are
ways where PBM could play a pivotal role optimizing biotechnological
and bioengineering applications.
OBJECTIVES
Here we investigated whether different wavelengths (blue,
green and red) would promote distinct outcomes in human
adipose-derived stem cells (hADSCs) cultured in regular and
supplemented media for tenocyte differentiation.
MATERIALS AND METHODS
Freshly isolated hADSCs were cultured in a specific stem cell
medium (MSCGM, Lonza), DMEM or a tenogenic medium
(TEN-M: DMEM supplemented with growth factors and ascorbic
acid). Cells were irradiated every 48 h (23.28 mW/cm 2 ,
17 min 10 s delivering 24 J/cm 2 per session) using a LED irradiator
(LEDbox, BioLambda). MTT and crystal violet assays
were used to evaluate cell metabolic activity and proliferation.
DISCUSSION AND RESULTS
Red wavelength (660 nm) significantly increased metabolic
activity after five irradiations, but only for cells cultured in
TEN-M. Oppositely, blue (450 nm) and green (520 nm) light
decreased both cell proliferation and metabolic rate, with more
pronounced effects for blue light in TEN-M. Considering these
findings, we examined whether irradiating only the media would generate toxic compounds that could impair cell viability. We
therefore assessed reactive oxygen species (ROS) production
by p-nitrosodimethylaniline/histidine assay while irradiating
the three different media under the same conditions as mentioned
above. Immediately after blue and green light exposure,
an increment in ROS production was observed for DMEM and
TEN-M, that continuously increased until reaching between 4.5
and 7.1 ??M one-hour after irradiation ??? with higher values for
TEN-M exposed to blue light.
CONCLUSION
Since no significant ROS formation was observed following
red light exposure, we concluded that medium composition
was responsible for the different effects on metabolic activity
and proliferation observed after irradiation with different
wavelengths. | |
| dc.format | 1492-1493 | |
| dc.relation | Biophysical Reviews | |
| dc.rights | openAccess | |
| dc.source | IUPAB International Congress, 20th; SBBf Congress, 45th; Annual Meeting of SBBq, 50th, October 4-8, 2021, S??o Paulo, SP | |
| dc.title | Low power light triggers opposite effects on stem cells | |
| dc.type | Resumos em peri??dicos | |
| dc.coverage | I | |
