| dc.creator | SOUZA, TIAGO H. dos S. | |
| dc.creator | ANDRADE, CAMILA | |
| dc.creator | CABRAL, FERNANDA | |
| dc.creator | SARMENTO-NETO, JOSE | |
| dc.creator | REBOUCAS, JULIO | |
| dc.creator | SANTOS, BEATE | |
| dc.creator | RIBEIRO, MARTHA | |
| dc.creator | FIGUEIREDO, REGINA | |
| dc.creator | FONTES, ADRIANA | |
| dc.date | 2021 | |
| dc.date | 2022-03-29T17:48:25Z | |
| dc.date | 2022-03-29T17:48:25Z | |
| dc.date.accessioned | 2023-09-28T14:21:42Z | |
| dc.date.available | 2023-09-28T14:21:42Z | |
| dc.identifier | 1867-2450 | |
| dc.identifier | http://repositorio.ipen.br/handle/123456789/32886 | |
| dc.identifier | 6 | |
| dc.identifier | 13 | |
| dc.identifier | 10.1007/s12551-021-00845-2 | |
| dc.identifier | 0000-0002-4203-1134 | |
| dc.identifier | Sem Percentil | |
| dc.identifier | 90.00 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/9003105 | |
| dc.description | INTRODUCTION
Photodynamic inactivation (PDI) has been attracting attention as an innovative
technology to treat topical diseases, such as cutaneous leishmaniasis
(CL) and infections caused by multidrug-resistant microorganisms.
Zn(II) meso-tetrakis(N-n-hexylpyridinium-2-yl)porphyrin
(ZnTnHex-2-PyP 4+ ) is a lipophilic water-soluble Zn(II) porphyrin with
improved photophysical properties, high chemical stability, and cationic/
amphiphilic character that can enhance its interaction with cells.
OBJECTIVES
Thus, this study aimed to investigate the PDI effects mediated by
ZnTnHex-2-PyP 4+ on Leishmania amazonensis.
MATERIALS AND METHODS
Confocal fluorescence microscopy was explored to study the interaction
of ZnTnHex-2-PyP 4+ with promastigotes. The PDI action was analyzed
by cell membrane integrity, mitochondrial membrane potential (????m),
and cell morphology. Promastigotes were incubated with
ZnTnHex-2-PyP 4+ for 5 min at 0.62 and 1.25 ??M and irradiated by a
LED (410 nm) for 1 or 3 min (2.3 and 3.4 J/cm 2 , respectively). PDI on
amastigotes and the cytotoxicity onmacrophages were also analyzed (3.4
J/cm 2 ).
DISCUSSION AND RESULTS
Fluorescence microscopy revealed that parasites efficiently uptake
ZnTnHex-2-PyP 4+ and displayed a punctate labeling pattern along with
the cytoplasm. An intense????mdepolarization was also observed, which
in association with microscopy results, suggests that ZnTnHex-2-PyP 4+
may accumulate in the mitochondrion, or other well-defined structures
close to it. Moreover, ZnTnHex-2-PyP 4+ at concentration as low as
0.62 ??M led to the immediate inactivation of >95% of promastigotes,
regardless of the light dose used. Loss of the fusiform shape and plasma
membrane wrinkling were also observed. After a single treatment session
in amastigotes, PDI led to a reduction of 70% in the infection index. No
considerable toxicity was observed on mammalian cells.
CONCLUSION
Thus, PDI of Leishmania parasites showed in vitro efficiency at a
submicromolar concentration of ZnTnHex-2-PyP 4+ , with short
pre-incubation and irradiation times. The results encourage further studies
in CL pre-clinical assays and PDI of other microorganisms. | |
| dc.format | 1380-1381 | |
| dc.relation | Biophysical Reviews | |
| dc.rights | openAccess | |
| dc.source | IUPAB International Congress, 20th; SBBf Congress, 45th; Annual Meeting of SBBq, 50th, October 4-8, 2021, S??o Paulo, SP | |
| dc.title | Effects of photodynamic inactivation mediated by Zn(II) porphyrin on promastigote and amastigote forms of Leishmania amazonensis | |
| dc.type | Resumos em peri??dicos | |
| dc.coverage | I | |
