dc.creator | MITSUTAKE, HERY | |
dc.creator | SOUZA, ALESSANDRO D. de | |
dc.creator | YOKAICHIYA, FABIANO | |
dc.creator | FRANCO, MARGARETH K.K.D. | |
dc.creator | CLEMENS, DANIEL | |
dc.creator | KENT, BEN | |
dc.creator | BREITKREITZ, MARCIA C. | |
dc.creator | RIBEIRO, LIGIA N. de M. | |
dc.creator | PAULA, ENEIDA de | |
dc.creator | BORDALLO, HELOISA N. | |
dc.date | 2021 | |
dc.date | 2022-03-29T17:39:52Z | |
dc.date | 2022-03-29T17:39:52Z | |
dc.date.accessioned | 2023-09-28T14:21:42Z | |
dc.date.available | 2023-09-28T14:21:42Z | |
dc.identifier | 1867-2450 | |
dc.identifier | http://repositorio.ipen.br/handle/123456789/32884 | |
dc.identifier | 6 | |
dc.identifier | 13 | |
dc.identifier | 10.1007/s12551-021-00845-2 | |
dc.identifier | Sem Percentil | |
dc.identifier | 90.00 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/9003103 | |
dc.description | INTRODUCTION
Local anesthetics are substances that reversibly block the nerve-impulse
conduction, alleviating pain without loss of consciousness. Benzocaine, a
poorly soluble local anesthetic, is an ester of para-aminobenzoic acid.
Several strategies of formulations can be used to improve bioavailability
and decrease adverse effects of benzocaine. In this study nanostructured
lipid carriers (NLC) were employed. These lipid-based drug delivery
carriers have a lipid core composed of a blend of solid and liquid lipids,
and a shelf of non-ionic surfactant.
OBJECTIVES
The main aim of this work was to optimize benzocaine-loaded NLC and
to investigate structural changes in these nanoparticles, under different
temperatures.
MATERIALS AND METHODS
The ratio of excipients (cetyl palmitate, Capmul?? PG-8 NF and
Pluronic??F68) and benzocaine in the NLC was optimized using a 2 3
factorial design with respect to the following parameters: particle size,
polydispersity index (PDI) and zeta potentials.
DISCUSSION AND RESULTS
The interactions between the factors were found relevant to determine
particle size and PDI. Using desirability function, the best formulation
conditions were found. Structural changes in optimized NLC were observed
with Small-Angle Neutron Scattering (SANS) and Raman imaging,
in samples at 27, 37 and 40?? C. SANS pointed the formation of
lamellar structures inside the NLC, which interlamellar distances increase
at higher temperature. Raman imaging showed that the incorporation of
P68 and benzocaine in-between the lipids increased at higher temperatures,
explaining the changes in Q values (SANS).
CONCLUSION
This work shows how different scattering techniques can provide complementary
information and be used together to characterize and understand
the physical, chemical, and structural changes on the organization
of pharmaceutical carriers in drug delivery system. | |
dc.format | 1335-1335 | |
dc.relation | Biophysical Reviews | |
dc.rights | openAccess | |
dc.source | IUPAB International Congress, 20th; SBBf Congress, 45th; Annual Meeting of SBBq, 50th, October 4-8, 2021, S??o Paulo, SP | |
dc.title | Evaluation of structural changes of benzocaine-loaded, optimized nanostructured lipid carriers using SANS and Raman imaging approaches | |
dc.type | Resumos em peri??dicos | |
dc.coverage | I | |