POLYAna is a new executable software developed by SISLIBIO group for rheological analysis of hydrogel and organogel systems and other colloidal materials (nanoparticles and micelles). The software development aims to facilitate the analysis of rheology data associated to both temperature- and frequency-dependent analysis, viscosity and curve flow profiles. The software development aims to facilitate the analysis of rheology data associated to both temperature- and frequency-dependent analysis, viscosity and curve flow profiles. From raw data, several models are applied like power-law model for frequency response and curve flow, Boltzmann law to calculate gelation temperature and viscosity response under temperature, Maxwell model to study interchain relationships in addition to other models such as Bingham model, Cross model, and Herschel-Bulkley are also available. POLYana outputs calculates rheological parameters like consistency, adhesion, hysteresis, flow index, G???/G?????? ratio. To validate results obtained from POLYana, same data were analyzed by applying other programs and same mathematical models. In this sense, rheological analysis of Poloxamer 407 in water solution (15 %) were performed: from temperature-dependent G??? and G?????? analysis were obtained gelation temperature of 45.46 ?? 0.02 ??C, ??_0 = 0.08 ?? 0.03 mPa*s, ??_max = (32.44 ?? 0.17) mPa*s and d??/dT = (1.27 ?? 0.02) mPa*s/??C by fitting Boltzmann law (R2 = 0.998), which are similar to results obtained by others softwares and found in literature. From temperature-dependent G??? and G?????? analysis, it gets adhesion value of (1647.15 ?? 18.01) mPa*sn calculated from power-law model (R2 = 0.869), also similar to PRISM results. Also, other Poloxamer concentrations and hydrogels types have been evaluated, showing close numbers to that previously reported. In order to stablish structural relationships, one of POLYana tools is also to analyze small-angle neutron scattering (SANS) and develop Monte Carlo simulation for SANS and rheological analysis, simultaneously.Coordena????o de Aperfei??oamento de Pessoal de N??vel Superior (CAPES)Conselho Nacional de Desenvolvimento Cient??fico e Tecnol??gico (CNPq)CAPES: 001CNPq: 307718/2019-0