Conservation and preservation strategies are essential to manage botanical collections specially for dried herbarium specimens also known as exsiccates, usually referring to a set of identified specimens belonging to taxa and distributed among all herbaria around the world. Particularly, these collections are very sensitive to the attack of fungi and insects. In recent years, disinfection by ionizing radiation has become an effective strategy to preserve cultural heritage objects and archived materials with excellent results. In this work, the effects on color properties of gamma radiation on exsiccates samples were studied. Thus, two exsiccates, botanical pressed and dehydrated samples were selected from the Dom Bento Jos?? Pickel Herbarium (SPSF), situated at S??o Paulo (Brazil). These samples comes from Asteraceae and Solanaceae families and were collected in 1946 and 1984. The irradiation was performed at the Multipurpose Gamma Irradiation Facility at IPEN applying absorbed doses of 1 kGy, 6 kGy and 10 kGy, which are values of absorbed dose for disinfestation and disinfection. Results were analyzed using colorimetry with CIELAB color space scale and scanning electron microscopy. The results showed that there were no significant changes on colorimetric morphological properties of the samples.