The objective of experiment was determinate the radiation dose for disinfestation to mango Mangifera indica cv. Haden, infested by Anastrepha fraterculus larvae. For realization of the experiment, were collected fruits in the field, which were taking to Entomology laboratory where there was an infestation by the flies in cages during 72 hours period. Waited for the development of the larvae 5 and 8 days to infestation, after the mangos were irradiated in a Cobalt-60 source with doses of: 0(control), 50, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1.100, 1.200 and 1.300 Gy. After the irradiation, the fruits were placed in climate chamber with 25 ??5??C of temperature and 70??5% of relative humidity. Waited the larvae exit to out of the fruit until the transformation in pupae and adult stage. By the results obtained we can concluded that the lethal dose to larvae in mangoes infested with 5 and 8 days were 600 Gy and 1.000 Gy (0.6 and 1 kGy) respectively. The dose of 50 Gy prevented the total adult emergence for both treatments.