The Autonomic Nervous System (ANS) is responsible for regulating various physiological processes in the human body. The Heart Rate Variability (HRV) represents a measurement used in evaluating the modulation of the ANS in different physiological conditions such as stress, physical activity, sleep, metabolic alterations, and, also, pathological conditions. Physical activity results in important changes in the cardiovascular system, such as an increase in blood flow and a decrease of the peripheral vascular resistance. Monitoring the peripheral microcirculation represents an important aid in evaluating the general conditions of an individual. The Laser Speckle Contrast Analysis (LASCA) is a non-invasive optical resource developed that uses nonionizing radiation in the region of the infrared and is important in the diagnosis of problems regarding the peripheral microcirculation. This work aimed to implement a method to obtain the HRV through the peripheral microcirculation utilizing the Laser Speckle Contrast Analysis (LASCA) technique. A commercial LASCA equipment was used to obtain a face-video. A custom software was implemented to process the LASCA videos and obtain the HRV. A heart rate monitor (HRM) was also utilized to measure the HRV and the values were compared against the ones obtained with LASCA. The method had consistent results in obtaining both the pulsation and the HRV, making it possible for future studies to use such a technique.Funda????o de Amparo ?? Pesquisa do Estado de S??o Paulo (FAPESP)FAPESP: 17/21851-0; 18/03517-8