The aim of this study was evaluate Fe in blood of athletes from different modalities (judo athletes, cyclist and long distance runners) by X-ray Fluorescence methodology using portable equipment, as an alternative for clinical practice. The study showed the practicality and efficacy of using this methodology for successive clinical evaluations, during the preparation period of competitions, providing data that help in the elaboration of a balanced diet, as well as contribute to the proposal of new clinical evaluation protocols. In addition, the results emphasize the need to adopt differentiated diets for adequate iron intake as a function of sports activity.