In this study, the role of accelerated electron irradiation on the removal of profenofos in aqueous solution and in peas was investigated. The samples were irradiated with a Rhodotron E-beam accelerator with 10 MeV energy at doses of 0 (control), 4.6, 12 and 32 kGy. Irradiated vegetable samples were extracted using a QuEChERS protocol (quick, easy, cheap, effective, rugged, and safe), allowing analysis of pesticide traces. Quantification of profenofos in aqueous solution was performed by using GC-MS in SIM mode and GC-MS/MS in MRM mode in vegetable sample. Degradation of profenofos increased with the E-beam dose and was much more important in aqueous solution than in vegetable. In irradiated aqueous solution, one degradation product was detected.