Resumos em peri??dicos
Development of gelatin based scaffold by gamma radiation for application as platelet rich plasma support for wound treatment
Registro en:
2232-0822
2
7
aguardando
Autor
VARCA, JUSTINE P.R.O.
FERRARI, ANDRE
PIGNATA, DANILO R.
GUAZZELLI, STEFANE K.
KLINGBEIL, MARIA F.G.
VARCA, GUSTAVO H.C.
LUGAO, ADEMAR B.
MATHOR, MONICA B.
Resumen
Gelatin is a natural polymer originated from the collagen, and presents poor mechanical
properties, however it is a natural and biocompatible polymer, and collagen is the main component
of the extracellular matrix1. Poly-vynil (alcohol) is an artificial polymer with interesting
mechanical properties and biocompatibility. Such polymers have been largely scientific studied
for biomedical application2. Platelet Rich Plasma (PRP) has been widely scientific explored in
many medical fields in the last decades, especially in orthopedic area and in athletic treatments3.
Based on the PRP desired properties regarded to the tissue regeneration, the present work aimed
to develop a scaffold to support PRP release for wound treatment and study the influence of
different radiation doses on a scaffold formation to apply as support for PRP release for wound
treatment. In specific terms, the impact of radiation will be evaluated through physicochemical
and morphologic characteristics. In the study, two polymers of different characteristics were
applied, and scaffolds were prepared based on two formulations, one composed by gelatin (7%,
w/w) and PVA (5%, w/w), and the second by gelatin (10%, w/w) and PVA (5%, w/w). The
formulations were solubilized together in distilled water and heated up to 80 ??C under constant
stirring for 1 hour. Posteriorly, the blends were disposed in circular glass molds. The samples
were cooled at 4 ??C for at least 24 h and then irradiated at 15, 25 and 50 kGy. After irradiation,
the samples were frozen and freeze dried. The scaffolds were characterized in terms of structure
and morphology by mechanical assays, differential scanning calorimetry, scanning electron
microscopy, optical coherence tomography and infrared spectroscopy. In addition, platelet
adhesion and release, and cytotoxic assays were also performed. Samples irradiated at 15 kGy
presented pore size diameter of around 1.4 ??m and porosity of 54%, while samples irradiated at
25 kGy, presented pore size diameter of around 1.1 ??m and porosity of 49%. Optical coherence
tomography showed that gelatin control samples presented more superficial degradation as
irradiation dose increased, while PVA control sample presented higher integrity, indicating that
this polymer is less sensitive to gamma radiation. The system presented suitable mechanical
properties and the platelet adhesion and release assays showed that the scaffold presented adequate
pore size range to host and release the platelets, and non-cytotoxic to platelets, featuring adequate
properties to be applied as dressing for wound treatments.