Antimicrobial activity represents a challenge for scientific community. Polymer processing conditions impose temperature limitation for stability of biocide nanoparticles. In the present work, we evaluate ZnO synthesized in laboratory, utilizing Zn(NO3)26H2O as precursor. The main proposal is to improve the incorporation of this nanoparticle on SEBS/PP resulting on biocide material films. Before application on SEBS/PP, the nanoparticles was tested by reduction of forming colony units (CFU)(%), Dynamical Light Scattering (DLS) and Zeta potential. On CFU tests, all samples showed biocide properties. The samples with concentration of 1% showed the higher biocide effect on E.coli bacteria, in comparison of lower concentrations. The obtained material biocide was processed on SEBS/PP for obtaining of polymeric films with biocide activity and the new material was characterized by: forming colony units (CFU)(%), Differential Scanning Calorimetry (DSC), and Fourier Transformed Infrared (FTIR).Funda????o de Amparo ?? Pesquisa do Estado de S??o Paulo (FAPESP)FAPESP: 17/20177-3