The photodynamic therapy stands out, not only for the few adverse effects to the veterinary patient but also for presenting environmental safety, not inducing microbial resistance and reducing generation of residual drugs on products intended for human consumption. After understanding the full potential of this new therapeutic modality through the previous chapters, the purpose of this chapter is to cover the main aspects to be considered by veterinarians to move PDT to clinical practice. It will be presented how the light source and photosensitizers should be chosen as well as the application modes for both antimicrobial and cancer PDT. Biosafety will also be addressed.