Clinical, serological, and parasitological analysis of snakes naturally infected with Cryptosporidium serpentis

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorPaiva, Philipp Ricardo S.O.
dc.creatorGrego, Kathleen F.
dc.creatorLima, Valéria M.F.
dc.creatorNakamura, Alex A.
dc.creatorda Silva, Deuvânia C.
dc.creatorMeireles, Marcelo Vasconcelos
dc.date2014-05-27T11:30:41Z
dc.date2016-10-25T18:54:09Z
dc.date2014-05-27T11:30:41Z
dc.date2016-10-25T18:54:09Z
dc.date2013-09-16
dc.date.accessioned2017-04-06T02:38:59Z
dc.date.available2017-04-06T02:38:59Z
dc.identifierVeterinary Parasitology.
dc.identifier0304-4017
dc.identifier1873-2550
dc.identifierhttp://hdl.handle.net/11449/76563
dc.identifierhttp://acervodigital.unesp.br/handle/11449/76563
dc.identifier10.1016/j.vetpar.2013.08.016
dc.identifierWOS:000327909600007
dc.identifier2-s2.0-84883732450
dc.identifier0000-0003-0063-5172
dc.identifierhttp://dx.doi.org/10.1016/j.vetpar.2013.08.016
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/897255
dc.descriptionInfection by Cryptosporidium serpentis is one of the most important diseases in reptiles and is characterized by chronic clinical or subclinical infection and the presence of hypertrophic gastritis, food regurgitation, progressive weight loss, mortality, and intermittent or continuous shedding of oocysts in the feces. The objectives of this study were to standardize an indirect enzyme-linked immunosorbent assay (ELISA) to detect antibodies against C. serpentis and to evaluate the clinical, parasitological, and humoral immune response in snakes naturally infected with C. serpentis. Twenty-one snakes naturally infected with C. serpentis and housed at the Butantan Institute, São Paulo, Brazil, underwent clinical and parasitological analyses for C. serpentis infection through daily records of clinical signs and a monthly survey of fecal shedding of oocysts using the Kinyoun's acid-fast staining. The serological evaluation was performed monthly by indirect ELISA using crude total antigen from oocysts of C. serpentis to detect anti-C. serpentis antibodies. Clinical symptoms consisted of food regurgitation, inappetence, and progressive weight loss. The parasitological analysis revealed intermittent fecal shedding of a variable number of oocysts in all snakes, with positivity in 85.32% (157/184) of the samples. The indirect ELISA was positive in 68.25% (86/126) of the samples. A humoral immune response was observed in most animals; however, fluctuating antibodies levels, leading to alternating positive and negative results, were observed in most snakes. © 2013 Elsevier B.V. All rights reserved.
dc.languageeng
dc.relationVeterinary Parasitology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCryptosporidium serpentis
dc.subjectDiagnostic
dc.subjectIndirect ELISA
dc.subjectSerology
dc.subjectSnakes
dc.titleClinical, serological, and parasitological analysis of snakes naturally infected with Cryptosporidium serpentis
dc.typeOtro


Este ítem pertenece a la siguiente institución