Artigo
Statistical experimental design to assess the influence of enzymes of nematophagous fungi versus helminths
Autor
Braga, Fabio Ribeiro
Soares, Filippe Elias de Freitas
Araujo, Juliana Milani
Fonseca, Leandro Abreu da
Hiura, Emy
Gava, Maylla Garschagen
Vieira, Fernanda Toledo
Paz, Jeanne Saraiva da
Carvalho, Lorendane Milena de
Faccini, João Victor
Queiroz, José Humberto de
Araújo, Jackson Victor
Institución
Resumen
The present work used Plackett–Burman experimental design to assess the influence of enzymes of nematophagous fungi versus Strongyloides westeri and trichostrongylides larvae and Platynosomum fastosum eggs. The variables studied in the Plackett–Burman design were the proteases and chitinases of AC001 or VC4 as destructive agents of S. westeri and trichostrongylides larvae, and P. fastosum eggs. All tested enzymes had a significant effect (P < 0.05) on the destruction of S. westeri larvae. Furthermore, only VC4 and AC001 proteases showed a significant effect (P < 0.05) on the destruction of trichostrongylides larvae. On the other hand, chitinases of VC4 showed the highest significance (P < 0.05) on the destruction of P. fastosum eggs. It is proposed that statistical planning for the use of enzymes derived from nematophagous fungi is a viable way to elucidate some questions about their mechanism of action.