Inheritance of coffee leaf rust resistance and identification of AFLP markers linked to the resistance gene

dc.creatorBrito, Giovani Greigh de
dc.creatorCaixeta, Eveline Teixeira
dc.creatorGallina, Ana Paula
dc.creatorZambolim, Eunize Maciel
dc.creatorZambolim, Laércio
dc.creatorDiola, Valdir
dc.creatorLoureiro, Marcelo Elhers
dc.date2018-08-30T10:47:58Z
dc.date2018-08-30T10:47:58Z
dc.date2010-03
dc.date.accessioned2023-09-27T22:04:40Z
dc.date.available2023-09-27T22:04:40Z
dc.identifier15735060
dc.identifierhttps://doi.org/10.1007/s10681-010-0119-x
dc.identifierhttp://www.locus.ufv.br/handle/123456789/21527
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8970355
dc.descriptionThe most important disease of Coffea arabica is coffee leaf rust caused by the fungus Hemileia vastatrix. The purpose of this study was to characterize the inheritance of coffee resistance gene(s) to race II of this pathogen and to identify and map molecular markers linked to this trait. Different populations were used: F2 (160 plants), BCr (20), and BCs (135), derived from a cross between the resistant genotype Híbrido de Timor UFV 427-15 and the susceptible cultivar Catuaí Amarelo UFV 2143-236 (IAC 30). The segregation analysis showed that the resistance of Híbrido de Timor to race II of the H. vastatrix is conferred by a single dominant gene. The amplification of 176 AFLP (Amplified fragment length polymorphism) primer combinations using bulked segregant analysis (BSA) allowed the identification of three molecular markers linked to the resistance gene. Genetic mapping of these three markers in the F2 population indicated that they are distributed on both sides, flanking the resistance gene. The markers E.CTC/M.TTT405 and E.CGT/M.TGT300 were found linked to the resistance gene at 8.69 cM (LOD 18.91) and 25.10 cM (LOD 5.37), respectively, while E.CCT/M.TTC230 was localized on the other side of the gene, at 20.50 cM (LOD 6.15). These markers are the first rust resistance markers identified in Híbrido de Timor and can be useful for marker assisted selection in coffee breeding programs.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherEuphytica
dc.relationv. 173, n. 2, p. 255– 264, may 2010
dc.rightsSpringer Science+Business Media B.V.
dc.subjectCoffee leaf rust
dc.subjectResistance gene
dc.subjectAFLP marker
dc.subjectBulked segregant analysis
dc.titleInheritance of coffee leaf rust resistance and identification of AFLP markers linked to the resistance gene
dc.typeArtigo


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