Effects of BST and high energy diet on gene expression in mammary parenchyma of dairy heifers

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorLew, Betina Joyce
dc.creatorde Oliveira, Mauro Dal Secco
dc.creatorJúnior, José Esler de Freitas
dc.creatorde Carvalho, Marina Vieira
dc.creatordo Rêgo, Aníbal Coutinho
dc.creatorRennó, Francisco Palma
dc.date2014-05-27T11:29:53Z
dc.date2016-10-25T18:50:48Z
dc.date2014-05-27T11:29:53Z
dc.date2016-10-25T18:50:48Z
dc.date2013-07-01
dc.date.accessioned2017-04-06T02:29:47Z
dc.date.available2017-04-06T02:29:47Z
dc.identifierRevista Brasileira de Zootecnia, v. 42, n. 7, p. 511-520, 2013.
dc.identifier1516-3598
dc.identifier1806-9290
dc.identifierhttp://hdl.handle.net/11449/75854
dc.identifierhttp://acervodigital.unesp.br/handle/11449/75854
dc.identifier10.1590/S1516-35982013000700008
dc.identifierS1516-35982013000700008
dc.identifierWOS:000322317000008
dc.identifier2-s2.0-84880705271.pdf
dc.identifier2-s2.0-84880705271
dc.identifierhttp://dx.doi.org/10.1590/S1516-35982013000700008
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/896584
dc.descriptionThe objective of this study was to determine the effects of dietary energy and recombinant bovine somatotropin (bST) injection to identify genes that might control mammogenesis. Total RNA was extracted from the parenchymal tissue of 32 heifers randomly assigned to one of four treatments: two diets (a standard diet and a high energy, high protein diet), each with or without bST. To perform microarray experiments, RNA samples were pooled (2 animals/pool) before reverse transcription and labeling with Cy3 or Cy5. A 4-node loop design was used to examine the differential gene expression among treatments using a bovine-specific cDNA micro array (National Bovine Functional Genomics Consortium Library, NBFGC) containing 18,263 unique expressed sequence tags (EST). Significance levels of differential gene expression among treatments were assessed using a mixed model approach. Injection of bST altered the expression of 12 % of the genes on NBFGC slide related to tissue development, whereas 6% were altered by diet. Administration of bST increases the expression of genes positively related to cell proliferation and mammary parenchyma to a greater extent than a high energy diet. © 2013 Sociedade Brasileira de Zootecnia.
dc.languageeng
dc.relationRevista Brasileira de Zootecnia
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGene regulation
dc.subjectMammary gland development
dc.subjectMicroarray
dc.subjectNutrition
dc.subjectSomatotropin
dc.titleEffects of BST and high energy diet on gene expression in mammary parenchyma of dairy heifers
dc.typeOtro


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