Produção de glicose e frutose por invertase de Saccharomyces cerevisiae imobilizada em suporte MANAE-agarose

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorGoulart, Antonio José
dc.creatorFrancisco dos Santos, Andréa
dc.creatorTavano, Olga Luisa
dc.creatorCésar Vinueza, Julio
dc.creatorContiero, Jonas
dc.creatorMonti, Rubens
dc.date2014-05-27T11:29:47Z
dc.date2016-10-25T18:50:12Z
dc.date2014-05-27T11:29:47Z
dc.date2016-10-25T18:50:12Z
dc.date2013-06-27
dc.date.accessioned2017-04-06T02:28:13Z
dc.date.available2017-04-06T02:28:13Z
dc.identifierRevista de Ciencias Farmaceuticas Basica e Aplicada, v. 34, n. 2, p. 169-175, 2013.
dc.identifier1808-4532
dc.identifierhttp://hdl.handle.net/11449/75722
dc.identifierhttp://acervodigital.unesp.br/handle/11449/75722
dc.identifier2-s2.0-84879254913.pdf
dc.identifier2-s2.0-84879254913
dc.identifierhttp://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/2570
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/896458
dc.descriptionInvertase from Saccharomyces cerevisiae was immobilized on agarose beads, activated with various groups (glyoxyl, MANAE or glutaraldehyde), and on some commercial epoxy supports (Eupergit and Sepabeads). Very active and stable invertase derivatives were produced by the adsorption of the enzyme on MANAE-agarose, MANAE-agarose treated with glutaraldhyde and glutaraldehyde-agarose supports. At pH 5.0, these derivatives retained full activity after 24h at 40°C and 50 °C. When assayed at 40°C and 50°C, with the pH adjusted to 7.0, the invertase-MANAE-agarose derivative treated with glutaraldehyde retained 80% of the initial activity. Recovered activities of the derivatives produced with MANAE, MANAE treated with glutaraldehyde and glutaraldehyde alone were 73.5%, 44.4% and 36.8%, respectively. These three preparations were successfully employed to produce glucose and fructose in 3 cycles of sucrose hydrolysis.
dc.languageeng
dc.languagepor
dc.relationRevista de Ciências Farmacêuticas Básica e Aplicada
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGlucose and fructose production
dc.subjectInvertase
dc.subjectInvertase immobilization
dc.subjectSaccharomyces cerevisiae
dc.subjectagarose
dc.subjectbeta fructofuranosidase
dc.subjectepoxy resin
dc.subjectfructose
dc.subjectglucose
dc.subjectglutaraldehyde
dc.subjectsucrose
dc.subjectadsorption
dc.subjectbiotechnological production
dc.subjectenzyme activity
dc.subjectenzyme assay
dc.subjectenzyme immobilization
dc.subjectenzyme stability
dc.subjecthydrolysis
dc.subjectkinetics
dc.subjectnonhuman
dc.subjectpH
dc.subjecttemperature
dc.subjectthermostability
dc.titleProdução de glicose e frutose por invertase de Saccharomyces cerevisiae imobilizada em suporte MANAE-agarose
dc.typeOtro


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