Development of a selective culture medium for bifidobacteria, Raffinose-Propionate Lithium Mupirocin (RP-MUP) and assessment of its usage with Petrifilm™ Aerobic Count plates

dc.creatorMiranda, Rodrigo Otávio
dc.creatorCarvalho, Antonio Fernandes de
dc.creatorNero, Luís Augusto
dc.date2019-01-30T17:40:58Z
dc.date2019-01-30T17:40:58Z
dc.date2014-05
dc.date.accessioned2023-09-27T21:38:49Z
dc.date.available2023-09-27T21:38:49Z
dc.identifier0740-0020
dc.identifierhttps://doi.org/10.1016/j.fm.2013.11.010
dc.identifierhttp://www.locus.ufv.br/handle/123456789/23262
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8964005
dc.descriptionThis study aimed to develop a selective culture media to enumerate bifidobacteria in fermented milk and to assess this medium when used with Petrifilm™ AC plates. For this purpose, Bifidobacterium spp., Lactobacillus spp. and Streptococcus thermophilus strains were tested to verify their fermentation patterns for different carbohydrates. All bifidobacteria strains were able to use raffinose. Based on these characteristic, a selective culture medium was proposed (Raffinose-Propionate Lithium Mupirocin, RP-MUP), used with Petrifilm™ AC plates, and was used to enumerate bifidobacteria in fermented milk. RP-MUP performance was assessed by comparing the results with this medium to reference protocols and culture media for bifidobacteria enumeration. RP-MUP, whether used or not with Petrifilm™ AC, presented similar performance to TOS-MUP (ISO 29981), with no significant differences between the mean bifidobacteria counts (p < 0.05) and with high correlation indices (r = 0.99, p < 0.05). As an advantage, reliable results were obtained after just 48 h of incubation when RP-MUP was used with Petrifilm™ AC, instead of the 72 h described in the ISO 29981 protocol.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherFood Microbiology
dc.relationVolume 39, Pages 96-102, May 2014
dc.rightsOpen Access
dc.subjectBifidobacteria
dc.subjectSelective enumeration
dc.subjectPetrifilm™ AC
dc.subjectTOS-MUP
dc.titleDevelopment of a selective culture medium for bifidobacteria, Raffinose-Propionate Lithium Mupirocin (RP-MUP) and assessment of its usage with Petrifilm™ Aerobic Count plates
dc.typeArtigo


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