Otro
CDKN2A promoter hypermethylation in astrocytomas is associated with age and sex
Registro en:
International Journal of Surgery, v. 11, n. 7, p. 549-553, 2013.
1743-9191
1743-9159
10.1016/j.ijsu.2013.05.030
WOS:000321750800011
2-s2.0-84880329284
2-s2.0-84878686126
Autor
Alves, Markênia Kélia Santos
Faria, Mário Henrique Girão
Neves Filho, Eduardo Henrique Cunha
Ferrasi, Adriana Camargo
Pardini, Maria Ines de Moura Campos
Moraes Filho, Manoel Odorico de
Rabenhorst, Silvia Helena Barem
Resumen
CDKN2A promoter hypermethylation has been widely related to many cancers. In astrocytomas, although CDKN2A (p16INK4A protein) is often inactivated, there are still some controversial issues regarding the mechanism by which this alteration occurs. Thus, we analyzed a series of astrocytomas to assess the association between CDKN2A expression and methylation of grade I-IV tumors (WHO) and clinicopathological parameters. DNA extracted from formalin-fixed paraffin-embedded material of 93 astrocytic tumors was available for CDKN2A promoter methylation analysis and p16INK4A expression by methylation-specific PCR and immunohistochemistry, respectively. A strong negative correlation between nuclear and cytoplasmic immunostaining and CDKN2A promoter methylation was found. Additionally, a significant negative correlation between CDKN2A promoter methylation and age was observed; also, female patients had statistically more CDKN2A methylated promoters (p=0.036) than men. In conclusion, CDKN2A inactivation by promoter methylation is a frequent event in astrocytomas and it is related to the age and sex of patients. © 2013 Surgical Associates Ltd.