Determination of sildenafil and vardenafil by capillary zone electrophoresis using capacitively coupled contactless conductivity detection

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorRajh Vidal, Denis Tadeu
dc.creatorAugelli, Marcio Antonio
dc.creatorLucio Do Lago, Claudimir
dc.date2014-05-27T11:28:56Z
dc.date2016-10-25T18:47:29Z
dc.date2014-05-27T11:28:56Z
dc.date2016-10-25T18:47:29Z
dc.date2013-04-21
dc.date.accessioned2017-04-06T02:20:48Z
dc.date.available2017-04-06T02:20:48Z
dc.identifierAnalytical Methods, v. 5, n. 8, p. 2041-2045, 2013.
dc.identifier1759-9660
dc.identifier1759-9679
dc.identifierhttp://hdl.handle.net/11449/75141
dc.identifierhttp://acervodigital.unesp.br/handle/11449/75141
dc.identifier10.1039/c3ay26470j
dc.identifierWOS:000316960200021
dc.identifier2-s2.0-84879518357
dc.identifierhttp://dx.doi.org/10.1039/c3ay26470j
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/895889
dc.descriptionA method based on capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D) for determination of two important phosphodiesterase type-5 inhibitors (sildenafil and vardenafil) is introduced. The background electrolyte (BGE) consisted of an aqueous solution of 500 mmol L-1 acetic acid, and the capillary was previously treated with polybrene solution to prevent cationic analytes from adsorbing onto the inner surface. Although the analytes migrate in the counter flow, the total time is short. An instrument with two C4D detectors allowed a seamless transition from a fast method (less than one minute) but of low-efficiency using the first detector to a more efficient method using the second detector. The analysis of commercial tablets showed no significant difference between CE-C4D and HPLC methods. Conductivity detection is a well-known low selectivity detection scheme, which in conjunction with the high mobility of the co-ion in the BGE (hydroxonium) allows one to predict that other cationic analogues of sildenafil can also be detected. This is an interesting feature given the increasing number of compounds in this class. © 2013 The Royal Society of Chemistry.
dc.languageeng
dc.relationAnalytical Methods
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBackground electrolytes
dc.subjectCapacitively coupled contactless conductivity detection
dc.subjectCapillary zone electrophoresis
dc.subjectConductivity detection
dc.subjectDetection scheme
dc.subjectPhosphodiesterases
dc.subjectSeamless transition
dc.subjectSignificant differences
dc.subjectCapillary electrophoresis
dc.subjectDetectors
dc.titleDetermination of sildenafil and vardenafil by capillary zone electrophoresis using capacitively coupled contactless conductivity detection
dc.typeOtro


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