Microplate alamarblue assay for paracoccidioides susceptibility testing

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorde Paula e Silva, A. C. A.
dc.creatorOliveira, H. C.
dc.creatorSilva, J. F.
dc.creatorSangalli-Leite, F.
dc.creatorScorzoni, L.
dc.creatorFusco-Almeida, A. M.
dc.creatorMendes-Giannini, Maria José Soares
dc.date2014-05-27T11:28:46Z
dc.date2016-10-25T18:46:30Z
dc.date2014-05-27T11:28:46Z
dc.date2016-10-25T18:46:30Z
dc.date2013-04-01
dc.date.accessioned2017-04-06T02:18:39Z
dc.date.available2017-04-06T02:18:39Z
dc.identifierJournal of Clinical Microbiology, v. 51, n. 4, p. 1250-1252, 2013.
dc.identifier0095-1137
dc.identifier1098-660X
dc.identifierhttp://hdl.handle.net/11449/74985
dc.identifierhttp://acervodigital.unesp.br/handle/11449/74985
dc.identifier10.1128/JCM.02914-12
dc.identifierWOS:000316220400032
dc.identifier2-s2.0-84875846563.pdf
dc.identifier2-s2.0-84875846563
dc.identifier0000-0002-8059-0826
dc.identifierhttp://dx.doi.org/10.1128/JCM.02914-12
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/895744
dc.descriptionCLSI method M27-A3 is not available for use with dimorphic fungi, such as those of the Paracoccidioides genus. In this study, we developed a microdilution method and added the alamarBlue reagent to test the responses of Paracoccidioides brasiliensis and Paracoccidioides lutzii against amphotericin B and itraconazole antifungals. The test proved to be sensitive, practical, and inexpensive and can be used to monitor the activity of low-growth microorganisms and their response to various drugs. © 2013, American Society for Microbiology.
dc.languageeng
dc.relationJournal of Clinical Microbiology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectalamarBlue
dc.subjectamphotericin B
dc.subjectitraconazole
dc.subjectreagent
dc.subjectunclassified drug
dc.subjectantifungal activity
dc.subjectantifungal susceptibility
dc.subjectbroth dilution
dc.subjectcontrolled study
dc.subjectfungal strain
dc.subjecthuman
dc.subjectintermethod comparison
dc.subjectminimum inhibitory concentration
dc.subjectnonhuman
dc.subjectParacoccidioides
dc.subjectParacoccidioides brasiliensis
dc.subjectParacoccidioides lutzii
dc.subjectpriority journal
dc.subjectAmphotericin B
dc.subjectAntifungal Agents
dc.subjectHumans
dc.subjectItraconazole
dc.subjectMicrobial Sensitivity Tests
dc.subjectOxazines
dc.subjectStaining and Labeling
dc.subjectXanthenes
dc.titleMicroplate alamarblue assay for paracoccidioides susceptibility testing
dc.typeOtro


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