Expression of genes encoding cytosolic and endoplasmic reticulum HSP90 proteins in the aquatic fungus Blastocladiella emersonii

dc.creatorFietto, Luciano G.
dc.creatorPugliese, Luciana
dc.creatorGeorg, Raphaela C.
dc.creatorGomes, Suely L.
dc.date2018-11-19T16:09:02Z
dc.date2018-11-19T16:09:02Z
dc.date2008-03-31
dc.date.accessioned2023-09-27T21:10:08Z
dc.date.available2023-09-27T21:10:08Z
dc.identifier03781119
dc.identifierhttps://doi.org/10.1016/j.gene.2008.01.005
dc.identifierhttp://www.locus.ufv.br/handle/123456789/22568
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8955858
dc.descriptionHSP90 proteins are important molecular chaperones involved in multiple cellular processes. This work reports the characterization of cDNAs encoding two distinct HSP90 proteins (named HSP90A and HSP90B) from the chytridiomycete Blastocladiella emersonii. Deduced amino acid sequences of HSP90A and HSP90B exhibit signatures of the cytosolic and endoplasmic reticulum (ER) HSP90 proteins, respectively. A genomic clone encoding HSP90A was also characterized indicating the presence of a single intron of 184 bp interrupting the coding region, located near the amino-terminus of the protein. Expression of both HSP90A and HSP90B genes increases significantly during heat shock at 38 °C, with highest induction ratios observed in cells stressed during germination of the fungus. Changes in the amount of HSP90A transcript were also evaluated during B. emersonii life cycle at physiological temperature (27 °C), and its levels were found to increase both during germination and sporulation of the fungus. HSP90A protein levels were analyzed during B. emersonii life cycle and significant changes were observed only during sporulation. Furthermore, during heat stress a large increase in the amount of HSP90A protein was observed. Induction of HSP90A and HSP90B genes during heat stress indicates the importance of both genes in the response to high temperature in B. emersonii.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherGene
dc.relationVolume 411, Issues 1– 2, Pages 59- 68, March 2008
dc.rights2008 Elsevier B.V. All rights reserved.
dc.subjectHeat shock
dc.subjectEndoplasmic reticulum HSP90
dc.subjectCytosolic HSP90
dc.subjectChytridiomycete
dc.titleExpression of genes encoding cytosolic and endoplasmic reticulum HSP90 proteins in the aquatic fungus Blastocladiella emersonii
dc.typeArtigo


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