Otro
Expression of annexin-A1 and galectin-1 anti-inflammatory proteins and mRNA in chronic gastritis and gastric cancer
Registro en:
Mediators of Inflammation, v. 2013.
0962-9351
1466-1861
10.1155/2013/152860
WOS:000314583500001
2-s2.0-84874527295.pdf
2-s2.0-84874527295
Autor
Jorge, Yvana Cristina
Mataruco, Mayra Mioto
Araújo, Leandro Pires
Rossi, Ana Flávia Teixeira
De Oliveira, Juliana Garcia
Valsechi, Marina Curado
Caetano, Alaor
Miyazaki, Kenji
Fazzio, Célia Sebastiana de Jesus
Thomé, Jorge Alberto
Rahal, Paula
Oliani, Sonia Maria
Silva, Ana Elizabete
Resumen
Objective. The anti-inflammatory proteins annexin-A1 and galectin-1 have been associated with tumor progression. This scenario prompted us to investigate the relationship between the gene and protein expression of annexin-A1 (ANXA1/AnxA1) and galectin-1 (LGALS1/Gal-1) in an inflammatory gastric lesion as chronic gastritis (CG) and gastric adenocarcinoma (GA) and its association with H. pylori infection. Methods. We analyzed 40 samples of CG, 20 of GA, and 10 of normal mucosa (C) by the quantitative real-time PCR (qPCR) technique and the immunohistochemistry assay. Results. High ANXA1 mRNA expression levels were observed in 90% (36/40) of CG cases (mean relative quantification RQ = 4.26 ± 2.03) and in 80% (16/20) of GA cases (mean RQ = 4.38 ± 4.77). However, LGALS1 mRNA levels were high (mean RQ = 2.44 ± 3.26) in 60% (12/20) of the GA cases, while low expression was found in CG (mean RQ = 0.43 ± 3.13; P < 0.01). Normal mucosa showed modest immunoreactivity in stroma but not in epithelium, while stroma and epithelium displayed an intense immunostaining in CG and GA for both proteins. Conclusion. These results have provided evidence that galectin-1 and mainly annexin-A1 are overexpressed in both gastritis and gastric cancer, suggesting a strong association of these proteins with chronic gastric inflammation and carcinogenesis. © 2013 Yvana Cristina Jorge et al.