Effect of phosphoric acid on the degradation of human dentin matrix

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorTezvergil-Mutluay, A.
dc.creatorMutluay, M.
dc.creatorSeseogullari-Dirihan, R.
dc.creatorAgee, K. A.
dc.creatorKey, W. O.
dc.creatorScheffel, D. L S
dc.creatorBreschi, L.
dc.creatorMazzoni, A.
dc.creatorTjäderhane, L.
dc.creatorNishitani, Y.
dc.creatorTay, F. R.
dc.creatorPashley, D. H.
dc.date2014-05-27T11:27:30Z
dc.date2016-10-25T18:41:06Z
dc.date2014-05-27T11:27:30Z
dc.date2016-10-25T18:41:06Z
dc.date2013-01-01
dc.date.accessioned2017-04-06T02:07:52Z
dc.date.available2017-04-06T02:07:52Z
dc.identifierJournal of Dental Research, v. 92, n. 1, p. 87-91, 2013.
dc.identifier0022-0345
dc.identifier1544-0591
dc.identifierhttp://hdl.handle.net/11449/74242
dc.identifierhttp://acervodigital.unesp.br/handle/11449/74242
dc.identifier10.1177/0022034512466264
dc.identifierWOS:000312209700015
dc.identifier2-s2.0-84870925270
dc.identifierhttp://dx.doi.org/10.1177/0022034512466264
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/895014
dc.descriptionThis study determined if dentin proteases are denatured by phosphoric acid (PA) used in etch-and-rinse dentin adhesives. Dentin beams were completely demineralized with EDTA for 30 days. We acid-etched experimental groups by exposing the demineralized dentin beams to 1, 10, or 37 mass% PA for 15 sec or 15 min. Control beams were not exposed to PA but were incubated in simulated body fluid for 3 days to assay their total endogenous telopeptidase activity, by their ability to solubilize C-terminal crosslinked telopeptides ICTP and CTX from insoluble dentin collagen. Control beams released 6.1 ± 0.8 ng ICTP and 0.6 ± 0.1 ng CTX/mg dry-wt/3 days. Positive control beams pre-incubated in p-aminophenylmercuric acetate, a compound known to activate proMMPs, released about the same amount of ICTP peptides, but released significantly less CTX. Beams immersed in 1, 10, or 37 mass% PA for 15 sec or 15 min released amounts of ICTP and CTX similar to that released by the controls (p > 0.05). Beams incubated in galardin, an MMP inhibitor, or E-64, a cathepsin inhibitor, blocked most of the release of ICTP and CTX, respectively. It is concluded that PA does not denature endogenous MMP and cathepsin activities of dentin matrices. © 2013 International & American Associations for Dental Research.
dc.languageeng
dc.relationJournal of Dental Research
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectbonding
dc.subjectcathepsins
dc.subjectcollagen
dc.subjectdemineralized
dc.subjectMMPs
dc.subject4 aminophenylmercuriacetate
dc.subject4-aminophenylmercuriacetate
dc.subjectcathepsin
dc.subjectcollagen type 1
dc.subjectcollagen type I trimeric cross linked peptide
dc.subjectcollagen type I trimeric cross-linked peptide
dc.subjectcollagenase
dc.subjectcysteine proteinase inhibitor
dc.subjectdipeptide
dc.subjectdrug derivative
dc.subjectenzyme activator
dc.subjectenzyme precursor
dc.subjectilomastat
dc.subjectleucine
dc.subjectmatrix metalloproteinase
dc.subjectmatrix metalloproteinase inhibitor
dc.subjectn [n (3 carboxyoxirane 2 carbonyl)leucyl]agmatine
dc.subjectpeptide
dc.subjectpeptide hydrolase
dc.subjectphenylmercuric acetate
dc.subjectphosphoric acid
dc.subjectthiol reagent
dc.subjectdentin
dc.subjectdrug antagonism
dc.subjectdrug effect
dc.subjectenzymology
dc.subjecthuman
dc.subjectmaterials testing
dc.subjectprotein denaturation
dc.subjecttime
dc.subjectCathepsins
dc.subjectCollagen Type I
dc.subjectCollagenases
dc.subjectCysteine Proteinase Inhibitors
dc.subjectDentin
dc.subjectDipeptides
dc.subjectEnzyme Activators
dc.subjectEnzyme Precursors
dc.subjectHumans
dc.subjectLeucine
dc.subjectMaterials Testing
dc.subjectMatrix Metalloproteinase Inhibitors
dc.subjectMatrix Metalloproteinases
dc.subjectPeptide Hydrolases
dc.subjectPeptides
dc.subjectPhenylmercuric Acetate
dc.subjectPhosphoric Acids
dc.subjectProtein Denaturation
dc.subjectSulfhydryl Reagents
dc.subjectTime Factors
dc.titleEffect of phosphoric acid on the degradation of human dentin matrix
dc.typeOtro


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