Adaptation of a silica membrane-based kit for purification of sequencing-ready BAC DNA

dc.creatorSakiyama, Ney S.
dc.creatorVallejos, C. Eduardo
dc.creatorJones, Valerie
dc.creatorShanker, Savita
dc.date2018-11-28T18:41:32Z
dc.date2018-11-28T18:41:32Z
dc.date2007-07-25
dc.date.accessioned2023-09-27T20:51:39Z
dc.date.available2023-09-27T20:51:39Z
dc.identifier15729818
dc.identifierhttp://dx.doi.org/10.1007/s11105-007-0001-8
dc.identifierhttp://www.locus.ufv.br/handle/123456789/22624
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8949595
dc.descriptionWe describe a modification of a protocol for the isolation of BAC DNA using a silica membrane-based kit designed for the isolation of plasmid DNA. The major advantages of this protocol are the expediency of the procedure, the high yield and purity, and the high quality of the BAC DNA that is suitable for direct sequencing.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherPlant Molecular Biology Reporter
dc.relationVolume 25, Issue 1– 2, Pages 11– 16, June 2007
dc.rightsSpringer-Verlag 2007
dc.subjectBAC DNA purification
dc.subjectDirect BAC DNA sequencing
dc.subjectBAC fingerprinting
dc.titleAdaptation of a silica membrane-based kit for purification of sequencing-ready BAC DNA
dc.typeArtigo


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