Enumeration of bifidobacteria using Petrifilm™ AC in pure cultures and in a fermented milk manufactured with a commercial culture of Streptococcus thermophilus

dc.creatorMiranda, Rodrigo Otávio
dc.creatorGama Neto, Gabriel
dc.creatorFreitas, Rosangela de
dc.creatorCarvalho, Antônio Fernandes de
dc.creatorNero, Luís Augusto
dc.date2018-05-11T14:33:28Z
dc.date2018-05-11T14:33:28Z
dc.date2011-07-21
dc.date.accessioned2023-09-27T20:40:06Z
dc.date.available2023-09-27T20:40:06Z
dc.identifier0740-0020
dc.identifierhttps://doi.org/10.1016/j.fm.2011.07.002
dc.identifierhttp://www.locus.ufv.br/handle/123456789/19508
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8946286
dc.descriptionBifidobacteria are probiotic microorganisms that are widely used in the food industry. With the aim of using of Petrifilm™ Aerobic Count (AC) plates associated with selective culture media, aliquots of sterile skim milk were inoculated separately with four commercial cultures of bifidobacteria. These cultures were plated by both the conventional method and Petrifilm™AC, using the culture media NNLP and ABC. The cultures were incubated under anaerobiosis at 37 °C for 24, 48 and 72 h. No significant differences (p > 0.05) were observed between the obtained counts at 48 and 72 h. Bifidobacteria counts in ABC were usually higher than in NNLP, independent of the plating method. Subsequently, fermented milk was prepared with a Streptococcus thermophilus strain, and aliquots were inoculated with the same bifidobacteria. Then, the fermented milks were submitted to microbiological analysis for bifidobacteria enumeration using the same culture media and methodologies previously described, incubated under anaerobiosis at 37 °C for 48 h. Again, bifidobacteria counts in ABC were higher than in NNLP, with significant differences for some cultures (p < 0.05). The counts obtained by both methodologies presented significant correlations (p < 0.05). The results indicate the viability of Petrifilm™AC as an alternative method for bifidobacteria enumeration when associated to specific culture media, specially the ABC.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherFood Microbiology
dc.relationVolume 28, Issue 8, Pages 1509-1513, December 2011
dc.rightsOpen Access
dc.subjectBifidobacteria
dc.subjectPetrifilm™AC
dc.subjectFermented milk
dc.titleEnumeration of bifidobacteria using Petrifilm™ AC in pure cultures and in a fermented milk manufactured with a commercial culture of Streptococcus thermophilus
dc.typeArtigo


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