Arabidopsis uses two gluconeogenic gateways for organic acids to fuel seedling establishment

dc.creatorEastmond, Peter J.
dc.creatorAstley, Holly M.
dc.creatorAubry, Sylvain
dc.creatorWilliams, Ben P.
dc.creatorMenard, Guillaume N.
dc.creatorCraddock, Christian P.
dc.creatorNunes-Nesi, Adriano
dc.creatorFernie, Alisdair R.
dc.creatorHibberd, Julian M.
dc.creatorParsley, Kate
dc.date2017-11-01T13:49:47Z
dc.date2017-11-01T13:49:47Z
dc.date2015-04-10
dc.date.accessioned2023-09-27T20:37:34Z
dc.date.available2023-09-27T20:37:34Z
dc.identifier2041-1723
dc.identifierhttp://dx.doi.org/10.1038/ncomms7659
dc.identifierhttp://www.locus.ufv.br/handle/123456789/12697
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8945233
dc.descriptionGluconeogenesis is a fundamental metabolic process that allows organisms to make sugars from non-carbohydrate stores such as lipids and protein. In eukaryotes only one gluconeogenic route has been described from organic acid intermediates and this relies on the enzyme phosphoenolpyruvate carboxykinase (PCK). Here we show that two routes exist in Arabidopsis, and that the second uses pyruvate, orthophosphate dikinase (PPDK). Gluconeogenesis is critical to fuel the transition from seed to seedling. Arabidopsis pck1 and ppdk mutants are compromised in seed-storage reserve mobilization and seedling establishment. Radiolabelling studies show that PCK predominantly allows sugars to be made from dicarboxylic acids, which are products of lipid breakdown. However, PPDK also allows sugars to be made from pyruvate, which is a major product of protein breakdown. We propose that both routes have been evolutionarily conserved in plants because, while PCK expends less energy, PPDK is twice as efficient at recovering carbon from pyruvate.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherNature Communications
dc.relation6:6659, Apr. 2015
dc.rightsOpen Access
dc.subjectArabidopsis
dc.subjectTwo gluconeogenic
dc.subjectFuel seedling
dc.titleArabidopsis uses two gluconeogenic gateways for organic acids to fuel seedling establishment
dc.typeArtigo


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