A simple spectrophotometry method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorRufino, José L.
dc.creatorPezza, Helena Redigolo
dc.creatorPezza, Leonardo
dc.date2014-05-27T11:25:21Z
dc.date2016-10-25T18:33:01Z
dc.date2014-05-27T11:25:21Z
dc.date2016-10-25T18:33:01Z
dc.date2010-12-01
dc.date.accessioned2017-04-06T01:47:41Z
dc.date.available2017-04-06T01:47:41Z
dc.identifierEcletica Quimica, v. 35, n. 4, p. 139-146, 2010.
dc.identifier0100-4670
dc.identifierhttp://hdl.handle.net/11449/72075
dc.identifierhttp://acervodigital.unesp.br/handle/11449/72075
dc.identifier10.1590/S0100-46702010000400018
dc.identifierS0100-46702010000400018
dc.identifier2-s2.0-80052606536.pdf
dc.identifier2-s2.0-80052606536
dc.identifier0000-0003-0197-7369
dc.identifierhttp://dx.doi.org/10.1590/S0100-46702010000400018
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/892982
dc.descriptionA simple, rapid, accurate and inexpensive spectrophotometric method for the determination of tetracycline and doxycycline has been developed. The method is based on the reaction between these drugs and chloramine-T in alkaline medium producing red color products with absorbance maximum at the λ=535 and 525 nm for the tetracycline and doxycycline, respectively. The best conditions for the reactions have been found using multivariate method. Beer's law is obeyed in a concentration ranges 1. 03 x 10 -5 to 3. 61 x 10 4mol L -1 and 1. 75 x 10 -5 to 3. 48 x 10 4mol L -1 for the tetracycline and doxycycline, respectively. The quantification limits were 5. 63 x 10 -6 mol L -1 and 7. 12 x 10 -7 mol L -1 for the tetracycline and doxycycline, respectively. The proposed method was successfully applied to the determination of these drugs in pharmaceutical formulations and the results obtained were in good agreement with those obtained by the comparative method at the 95% confidence level.
dc.languageeng
dc.relationEclética Química
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChloramine-T
dc.subjectDoxycycline
dc.subjectSpectrophotometric
dc.subjectTetracycline
dc.titleA simple spectrophotometry method for the determination of tetracycline and doxycycline in pharmaceutical formulations using chloramine-T
dc.typeOtro


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