info:eu-repo/semantics/article
COLLECT TRACHEAL SECRETION: A COMPARATIVE STUDY OF TECHNIQUES
COLETA DE SECREÇÃO TRAQUEAL: ESTUDO COMPARATIVO DE TÉCNICAS
Registro en:
10.5902/2236583411376
Autor
Serafim, Silvia Dubou
Souza, Juliana Alves
Soares, Janice Cristina
Dal Forno, Nara Lucia Frasson
Institución
Resumen
Objective: The objective of this study is to compare, by means of microbiological analysis, two techniques of tracheal aspirates in intubated or tracheostomized patients. Methodology: The study was conducted from May to June 2012, in HUSM at UFSM, becoming an experimental study, qualitative and quantitative, comparative trial. There were two data collection techniques in each hipersecretive patient, intubated or tracheostomized, requiring analysis of secretion, with the same random order. In technique 1 the secretion was aspirated until probe was with a sufficient amount of secretion, after the aspiration tube was cut with sterile scissors, into small pieces within the sterile flask. In technique 2, when the probe had a sufficient amount of secretion, it was clamped, the extender was disconnected and connected to the suction end of the oxygen flow meter. Following, the probe tip was placed into a sterile collection vial while the flow meter was connected to 15 l / min so that the secretion present inside the tube is pushed into the pot. Results: The bacteria most commonly found in the Intensive Care Unit were Pseudomonas aeruginosa (13.64%) and Acinetobacter baumannii (13.64%) also had the presence of Candida spp (18.18%). The relationship between both techniques is equal (Kappa coefficient), both as a Gram stain for bacteria. Conclusion: The microbiological analysis showed that the collection techniques with the tracheal secretion tube and cut the flow of oxygen are equivalent. Soon both can be used in hospital routine.Keywords: Infection, Artificial Respiration, Microbiological Analysis http://dx.doi.org/10.5902/2236583411376 Objetivo: Comparar, por meio de análise microbiológica, duas técnicas de coleta de secreção traqueal. Metodologia: estudo experimental, quantitativo, comparativo, cego, randomizado, desenvolvido na Unidade de Tratamento Intensivo (UTI). Foram realizadas duas técnicas de coleta em cada paciente hipersecretivo, entubado ou traqueostomizado, que necessitou de análise de secreção, sendo a ordem das mesmas aleatória. Na técnica 1 a sonda de aspiração foi cortada com tesoura estéril e colocada em pequenas parte dentro de frasco estéril. Já na técnica 2, a secreção que estava dentro da sonda de aspiração foi empurrada por fluxômetro de oxigênio para dentro do frasco estéril. Resultados: Os microorganismos mais encontrados na UTI foram Pseudomonas aeruginosa (13,64%), Acinetobacter baumanni (13,64%), e Candida spp (18,18%). A relação entre ambas às técnicas foi de igualdade (coeficiente Kappa), tanto para a De Gram como para a identificação das bactérias. Conclusão: A análise microbiológica demonstrou que as técnicas de coleta de secreção traqueal com a sonda cortada e com o fluxo de oxigênio são equivalentes. Logo ambas podem ser utilizadas na rotina hospitalar.Descritores: Infecção Hospitalar; Respiração Artificial; Análise Microbiológica.