Otro
Susceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode
Registro en:
Brazilian Oral Research, v. 24, n. 4, p. 413-418, 2010.
1806-8324
1807-3107
10.1590/S1806-83242010000400007
S1806-83242010000400007
2-s2.0-78650628907.pdf
2-s2.0-78650628907
Autor
da Costa, Anna Carolina Borges Pereira
Chibebe Junior, José
Pereira, Cristiane Aparecida
Machado, Ana Karina da Silva
Beltrame Junior, Milton
Junqueira, Juliana Campos
Jorge, Antonio Olavo Cardoso
Resumen
The objective of this study was to evaluate the effect of photodynamic therapy with erythrosine and rose bengal using a light-emitting diode (LED) on planktonic cultures of S. mutans. Ten S. mutans strains, including nine clinical strains and one reference strain (ATCC 35688), were used. Suspensions containing 10 6 cells/mL were prepared for each strain and were tested under different experimental conditions: a) LED irradiation in the presence of rose bengal as a photosensitizer (RB+L+); b) LED irradiation in the presence of erythrosine as a photosensitizer (E+L+); c) LED irradiation only (P-L+); d) treatment with rose bengal only (RB+L-); e) treatment with erythrosine only (E+L-); and f) no LED irradiation or photosensitizer treatment, which served as a control group (P-L-). After treatment, the strains were seeded onto BHI agar for determination of the number of colony-forming units (CFU/mL). The results were submitted to analysis of variance and the Tukey test (p ≤ 0.05). The number of CFU/mL was significantly lower in the groups submitted to photodynamic therapy (RB+L+ and E+L+) compared to control (P-L-), with a reduction of 6.86 log 10 in the RB+L+ group and of 5.16 log 10 in the E+L+ group. Photodynamic therapy with rose bengal and erythrosine exerted an antimicrobial effect on all S. mutans strains studied.