Morphologic changes and the expression of alpha-melanocyte stimulating hormone and melanocortin-1 receptor in melasma lesions: A comparative study

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMiot, Luciane D.
dc.creatorMiot, Hélio Amante
dc.creatorPolettini, Jossimara
dc.creatorSilva, Marcia Guimarães da
dc.creatorMarques, Mariângela Esther Alencar
dc.date2014-05-27T11:24:48Z
dc.date2016-10-25T18:29:07Z
dc.date2014-05-27T11:24:48Z
dc.date2016-10-25T18:29:07Z
dc.date2010-10-01
dc.date.accessioned2017-04-06T01:43:04Z
dc.date.available2017-04-06T01:43:04Z
dc.identifierAmerican Journal of Dermatopathology, v. 32, n. 7, p. 676-682, 2010.
dc.identifier0193-1091
dc.identifierhttp://hdl.handle.net/11449/71892
dc.identifierhttp://acervodigital.unesp.br/handle/11449/71892
dc.identifier10.1097/DAD.0b013e3181cd4396
dc.identifier2-s2.0-77957378083
dc.identifierhttp://dx.doi.org/10.1097/DAD.0b013e3181cd4396
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/892817
dc.descriptionMelasma is a common acquired symmetrical hypermelanosis characterized by irregular light- to dark-brown macules on sun-exposed skin areas. The literature shows few studies on its physiopathogeny. However, changes in α-melanocyte stimulating hormone (α-MSH) secretion and melanocortin-1 receptor (MC1-R) expression may play a role to trigger this condition. Biopsies were taken from both melasma skin and adjacent perilesional normal skin of 44 patients. The biopsies were submitted for hematoxylin and eosin and Fontana-Masson staining and immunohistochemistry with Melan-A, α-MSH, and MC1-R, and processed for transmission electron microscopy. In some cases, they were submitted to MC1-R gene expression analysis by real-time polymerase chain reaction. Increased lymphohistiocytic infiltrate and solar elastosis, higher epidermal melanin were observed in melasma skin. Electron microscopy revealed a greater number of mature melanosomes in keratinocytes and melanocytes, and more prominent cytoplasmic organelles in melasma skin. There was no difference in melanocyte number between areas. However, melanocytes were larger and more dendritic in melasma skin. Immunohistochemistry with α-MSH and MC1-R showed significant labeling in melasmic epidermis but MC1-R messenger ribonucleic acid (RNAm) did not show significant quantitative difference between melasma and normal skin. © 2010 by Lippincott Williams & Wilkins.
dc.languageeng
dc.relationAmerican Journal of Dermatopathology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectalpha-MSH
dc.subjectimmunohistochemistry
dc.subjectmelanosis
dc.subjectpolymerase chain reaction receptor
dc.subjecttype 1 melanocortin
dc.subjectalpha intermedin
dc.subjecteosin
dc.subjecthematoxylin
dc.subjectmelan A
dc.subjectmelanin
dc.subjectmelanocortin 1 receptor
dc.subjectmessenger RNA
dc.subjectcell organelle
dc.subjectchloasma
dc.subjectclinical article
dc.subjectcomparative study
dc.subjectcontrolled study
dc.subjectelastosis
dc.subjectgene expression
dc.subjecthuman
dc.subjectkeratinocyte
dc.subjectlymphocytic infiltration
dc.subjectmelanocyte
dc.subjectmelanosome
dc.subjectmorphology
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectquantitative analysis
dc.subjectreal time polymerase chain reaction
dc.subjectskin biopsy
dc.subjecttransmission electron microscopy
dc.subjectFemale
dc.subjectGene Expression
dc.subjectHumans
dc.subjectImage Processing, Computer-Assisted
dc.subjectImmunohistochemistry
dc.subjectMelanosis
dc.subjectMicroscopy, Electron, Transmission
dc.subjectReceptor, Melanocortin, Type 1
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.titleMorphologic changes and the expression of alpha-melanocyte stimulating hormone and melanocortin-1 receptor in melasma lesions: A comparative study
dc.typeOtro


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