Occurrence and expression of p53 suppressor gene and c-Myc oncogene in dog eyelid tumors

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorLopes, Rodrigo Antonio
dc.creatorCardoso, Tereza Cristina
dc.creatorLuvizotto, Maria Cecília Rui
dc.creatorAndrade, Alexandre Lima de
dc.date2014-05-27T11:24:38Z
dc.date2016-10-25T18:28:23Z
dc.date2014-05-27T11:24:38Z
dc.date2016-10-25T18:28:23Z
dc.date2010-03-01
dc.date.accessioned2017-04-06T01:40:39Z
dc.date.available2017-04-06T01:40:39Z
dc.identifierVeterinary Ophthalmology, v. 13, n. 2, p. 69-75, 2010.
dc.identifier1463-5216
dc.identifier1463-5224
dc.identifierhttp://hdl.handle.net/11449/71585
dc.identifierhttp://acervodigital.unesp.br/handle/11449/71585
dc.identifier10.1111/j.1463-5224.2009.00753.x
dc.identifier2-s2.0-77951605799
dc.identifierhttp://dx.doi.org/10.1111/j.1463-5224.2009.00753.x
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/892556
dc.descriptionPurpose: To detect the occurrence and expression of the suppressor gene p53 and of the oncogene c-Myc in eyelid tumors of dogs using the PCR, RT-PCR, PCR-ELISA and RT-PCR-ELISA techniques. These genes have not been described in dog eyelid tumors before. Methods: Nine samples of eyelid or third eyelid epithelial tumors were obtained from the archives of the Department of Veterinary Pathology. Tumor diagnosis was confirmed by evaluation of hematoxylin-eosin stained sections, and immunohistochemistry for cytokeratin AE1/AE3 and vimentin V9. A canine mammary tumor was used for positive control. Agarose gel electrophoresis, PCR-ELISA and RT-PCR-ELISA were used to detect p53 and c-Myc genes. Results: The occurrence of p53 was detected in most of the eyelid tumors and third eyelid tumors studied (88.8%, n = 8) and was expressed in 75% of the positive samples, as indicated by ELISA. The c-Myc gene was found in 77.7% (n = 7) of the samples and was expressed in eight samples. Conclusions: Eyelid and third eyelid tumors of dogs express both the p53 and the c-Myc genes as shown by PCR and RT-PCR. However, PCR ELISA and RT-PCR ELISA were more efficient in assessing occurrence and expression of these genes because they identified amplified products that were not detected by agarose gel electrophoresis. © 2010 American College of Veterinary Ophthalmologists.
dc.languageeng
dc.relationVeterinary Ophthalmology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectC-Myc
dc.subjectDog
dc.subjectELISA
dc.subjectEyelid tumors
dc.subjectP53
dc.subjectPCR
dc.subjectDNA
dc.subjectMyc protein
dc.subjectprotein p53
dc.subjectRNA
dc.subjectanimal
dc.subjectanimal disease
dc.subjectdog
dc.subjectdog disease
dc.subjecteyelid tumor
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectphysiology
dc.subjectAnimals
dc.subjectDog Diseases
dc.subjectDogs
dc.subjectEyelid Neoplasms
dc.subjectGene Expression Regulation, Neoplastic
dc.subjectProto-Oncogene Proteins c-myc
dc.subjectTumor Suppressor Protein p53
dc.subjectCanis familiaris
dc.titleOccurrence and expression of p53 suppressor gene and c-Myc oncogene in dog eyelid tumors
dc.typeOtro


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