First isolation of microorganisms from the gut diverticulum of Aedes aegypti (Diptera: Culicidae): New perspectives for an insect-bacteria association

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorGusmão, Desiely Silva
dc.creatorSantos, Adão Valmir
dc.creatorMarini, Danyelle Cristine
dc.creatorRusso, Érica de Souza
dc.creatorPeixoto, Anelise Maria Dias
dc.creatorBacci Jr., Maurício
dc.creatorBerbert-Molina, Marília Amorim
dc.creatorLemos, Francisco José Alves
dc.date2014-05-27T11:22:40Z
dc.date2016-10-25T18:24:41Z
dc.date2014-05-27T11:22:40Z
dc.date2016-10-25T18:24:41Z
dc.date2007-12-01
dc.date.accessioned2017-04-06T01:27:58Z
dc.date.available2017-04-06T01:27:58Z
dc.identifierMemorias do Instituto Oswaldo Cruz, v. 102, n. 8, p. 919-924, 2007.
dc.identifier0074-0276
dc.identifier1678-8060
dc.identifierhttp://hdl.handle.net/11449/70054
dc.identifierhttp://acervodigital.unesp.br/handle/11449/70054
dc.identifier10.1590/S0074-02762007000800005
dc.identifierS0074-02762007000800005
dc.identifierWOS:000252497600005
dc.identifier2-s2.0-38149127952.pdf
dc.identifier2-s2.0-38149127952
dc.identifierhttp://dx.doi.org/10.1590/S0074-02762007000800005
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/891209
dc.descriptionWe show for the first time that the ventral diverticulum of the mosquito gut (impermeable sugar storage organ) harbors microorganisms. The gut diverticulum from newly emerged and non-fed Aedes aegypti was dissected under aseptic conditions, homogenized and plated on BHI medium. Microbial isolates were identified by sequencing of 16S rDNA for bacteria and 28S rDNA for yeast. A direct DNA extraction from Ae. aegypti gut diverticulum was also performed. The bacterial isolates were: Bacillus sp., Bacillus subtilis and Serratia sp. The latter was the predominant bacteria found in our isolations. The yeast species identified was Pichia caribbica.
dc.languageeng
dc.relationMemórias do Instituto Oswaldo Cruz
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAedes aegypti
dc.subjectGut diverticulum
dc.subjectMicrobiota
dc.subjectSerratia sp.
dc.subjectbacterial DNA
dc.subjectribosome DNA
dc.subjectRNA 16S
dc.subjectRNA 28S
dc.subjectAedes
dc.subjectanimal
dc.subjectBacillus
dc.subjectgastrointestinal tract
dc.subjectgenetics
dc.subjectisolation and purification
dc.subjectmicrobiology
dc.subjectPichia
dc.subjectSerratia
dc.subjectAnimals
dc.subjectDNA, Bacterial
dc.subjectDNA, Ribosomal
dc.subjectGastrointestinal Tract
dc.subjectRNA, Ribosomal, 16S
dc.subjectRNA, Ribosomal, 28S
dc.subjectBacillus (bacterium)
dc.subjectBacillus sp.
dc.subjectBacillus subtilis
dc.subjectBacteria (microorganisms)
dc.subjectCulicidae
dc.subjectDiptera
dc.subjectHexapoda
dc.subjectPichia caribbica
dc.titleFirst isolation of microorganisms from the gut diverticulum of Aedes aegypti (Diptera: Culicidae): New perspectives for an insect-bacteria association
dc.typeOtro


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