Differential characterization of holocentric chromosomes in triatomines (Heteroptera, Triatominae) using different staining techniques and fluorescent in situ hybridization

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMorielle-Souza, A.
dc.creatorOliveira, Maria Tercília Vilela de Azeredo
dc.date2014-05-27T11:22:38Z
dc.date2016-10-25T18:24:33Z
dc.date2014-05-27T11:22:38Z
dc.date2016-10-25T18:24:33Z
dc.date2007-11-19
dc.date.accessioned2017-04-06T01:27:25Z
dc.date.available2017-04-06T01:27:25Z
dc.identifierGenetics and Molecular Research, v. 6, n. 3, p. 713-720, 2007.
dc.identifier1676-5680
dc.identifierhttp://hdl.handle.net/11449/69990
dc.identifierhttp://acervodigital.unesp.br/handle/11449/69990
dc.identifierWOS:000251696600028
dc.identifier2-s2.0-36048993706.pdf
dc.identifier2-s2.0-36048993706
dc.identifierhttp://www.geneticsmr.com//year2007/vol6-3/pdf/gmr0343.pdf
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/891152
dc.descriptionA comparative study of holocentric chromosomes in the triatomine species Panstrongylus megistus, Rhodnius pallescens and Triatoma infestans was carried out in order to characterize heterochromatin, rDNA active sites and nucleolar proteins. Cytological preparations of seminiferous tubules were stained by silver impregnation, C banding, fluorochromes CMA 3/DA and DAPI/DA, and fluorescent in situ hybridization (FISH) with Drosophila melanogaster 28S rDNA probe. Our results showed interesting aspects of the organization of chromatin and chromosomes in the meiotic cells of these insects. In R. pallescens, sex chromosomes (X, Y) were distinct from autosomes, when submitted to silver impregnation, C banding, CMA 3 staining, and FISH, confirming that these chromosomes bear nucleolar organizer regions (NORs). In P. megistus, two of the three sex chromosomes were CMA 3/DAPI-; at early meiotic prophase and at diakinesis, silver impregnation corresponded with FISH signals, indicating that in this species, two chromosomes (probably a sex chromosome and an autosome) bear NORs. In T. infestans, silver nitrate and FISH also stained corresponding areas on meiotic chromosomes. Our data suggest that in triatomines, in general, the number and location of NORs are species-specific. These regions may be considered important chromosome markers for comparative studies to improve the understanding of evolutionary mechanisms in these hematophagous insects. ©FUNPEC-RP.
dc.languageeng
dc.relationGenetics and Molecular Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCMA 3
dc.subjectDAPI
dc.subjectFluorescent in situ hybridization
dc.subjectHolocentric chromosomes
dc.subjectNucleolus organizer region
dc.subjectTriatomines
dc.subject4',6 diamidino 2 phenylindole
dc.subjectchromomycin A3
dc.subjectribosome DNA
dc.subjectribosome DNA 28s
dc.subjectsilver
dc.subjectunclassified drug
dc.subjectautosome
dc.subjectchromosome
dc.subjectchromosome analysis
dc.subjectchromosome marker
dc.subjectchromosome NOR
dc.subjectDNA probe
dc.subjectDrosophila melanogaster
dc.subjectfluorescence in situ hybridization
dc.subjectHeteroptera
dc.subjectholocentric chromosome
dc.subjectmeiosis
dc.subjectnonhuman
dc.subjectPanstrongylus
dc.subjectpanstrongylus megistus
dc.subjectprophase
dc.subjectRhodnius pallescense
dc.subjectsex chromosome
dc.subjectsignal detection
dc.subjectstaining
dc.subjectTriatoma infestans
dc.subjectTriatominae
dc.subjectAnimals
dc.subjectChromosomes
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectMale
dc.subjectRhodnius
dc.subjectSeminiferous Tubules
dc.subjectStaining and Labeling
dc.subjectTriatoma
dc.subjectHexapoda
dc.subjectPanstrongylus megistus
dc.subjectRhodnius pallescens
dc.titleDifferential characterization of holocentric chromosomes in triatomines (Heteroptera, Triatominae) using different staining techniques and fluorescent in situ hybridization
dc.typeOtro


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