Effects of basic fibroblast growth factor on density and morphology of fibroblasts grown on root surfaces with or without conditioning with tetracycline or EDTA.
Journal of oral science, v. 49, n. 3, p. 213-220, 2007.
Silvério, Karina G
Martinez, Aurora E T
Rossa Júnior, Carlos
A study was conducted to evaluate in vitro the effect of root surface conditioning with basic fibroblast growth factor (b-FGF) on morphology and proliferation of fibroblasts. Three experimental groups were used: non-treated, and treated with 50 microg or 125 microg b-FGF/ml. The dentin samples in each group were divided into subgroups according to the chemical treatment received before application of b-FGF: none, or conditioned with tetracycline-HCl or EDTA. After contact with b-FGF for 5 min, the samples were incubated for 24 h with 1 ml of culture medium containing 1 x 10(5) cells/ml plus 1 ml of culture medium alone. The samples were then subjected to routine preparation for SEM, and random fields were photographed. Three calibrated and blind examiners performed the assessment of morphology and density according to two index systems. Classification and regression trees indicated that the root surfaces treated with 125 microg b-FGF and previously conditioned with tetracycline-HCl or EDTA presented a morphology more suggestive of cellular adhesion and viability (P = 0.004). The density of fibroblasts on samples previously conditioned with EDTA, regardless of treatment with b-FGF, was significantly higher than in the other groups (P < 0.001). The present findings suggest that topical application of b-FGF has a positive influence on both the density and morphology of fibroblasts.
Showing items related by title, author, creator and subject.
Fibroblasts induce epithelial to mesenchymal transition in breast tumor cells which is prevented by fibroblasts treatment with histamine in high concentration Porretti, Juliana Carla; Mohamad, Nora A.; Martin, Gabriela Adriana; Cricco, Graciela P. (Elsevier, 2014-06)Epithelial to mesenchymal transition (EMT) of cancer cells is an essential process in cancer progression. Cancer cells that undergone EMT loose cell–cell contacts, acquire mesenchymal properties and develop migratory and ...
Expression of fibroblast growth factor 10 and its receptor, fibroblast growth factor receptor 2B, in the bovine corpus luteum CASTILHO, A. C.; GIOMETTI, I. C.; BERISHA, B.; SCHAMS, D.; PRICE, C. A.; AMORIM, R. L.; PAPA, P. C.; BURATINI JR., J. (WILEY-LISS, 2008)There is evidence that several fibroblast growth factors (FGFs) are involved in growth and development of the corpus luteum (CL), but many FGFs have not been investigated in this tissue, including FGF10. The objective of ...
Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts DAMANTE, Carla Andreotti; MICHELI, Giorgio De; MIYAGI, Sueli Patricia Harumi; FEIST, Iliria Salomao; MARQUES, Marcia Martins (SPRINGER LONDON LTD, 2009)The effects of laser phototherapy on the release of growth factors by human gingival fibroblasts were studied in vitro. Cells from a primary culture were irradiated twice (6 h interval), with continuous diode laser ...