Bio-safety technology in production of bovine herpesvirus type 5 (BoHV-5) using an alternative serum-free medium

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorCardoso, Tereza C.
dc.creatorFerrari, Heitor F.
dc.creatorLuvizotto, Maria Cecília R.
dc.creatorArns, Clarice W.
dc.date2014-05-27T11:22:30Z
dc.date2016-10-25T18:23:57Z
dc.date2014-05-27T11:22:30Z
dc.date2016-10-25T18:23:57Z
dc.date2007-06-04
dc.date.accessioned2017-04-06T01:25:15Z
dc.date.available2017-04-06T01:25:15Z
dc.identifierAmerican Journal of Biochemistry and Biotechnology, v. 3, n. 3, p. 125-130, 2007.
dc.identifier1553-3468
dc.identifierhttp://hdl.handle.net/11449/69713
dc.identifierhttp://acervodigital.unesp.br/handle/11449/69713
dc.identifier10.3844/ajbbsp.2007.125.130
dc.identifier2-s2.0-34249683333.pdf
dc.identifier2-s2.0-34249683333
dc.identifierhttp://dx.doi.org/10.3844/ajbbsp.2007.125.130
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/890919
dc.descriptionThe detection and growth of bovine herpes virus type 5 (BoHV-5) was evaluated in three different media without foetal bovine serum or animal protein by infecting three different cell lines. The OPTI-PRO, VP-SFM and RPMI 1640 media were supplemented by L-glutamine, antibiotics added by 5μg of insulin-like growth factor (IGF-I) and used to adapt CER, MDBK and CRIB cells in a statistic continuous culture system. The results obtained by CRIB and MDBK cells adaptation showed steady growth after 10th passages in OPTI-PRO medium, 20th passages in VP-SFM medium and 30th passages in RPMI 1640 medium, respectively. The OPTI-PRO and RPMI 1640 media were not able to support CER growth, even supplemented by IGF-1 and present apoptotic cells at 72h post-infection. The CER cells seeded after adaptation by 10 th passages in VP-SFM medium added by 5μg/20ml of IGF-1 growth factor revealed the best virus titres compared to MDBK and CRIB cell lines. It was concluded that CER, MDBK and CRIB cells infected by BoHV-5 serotype could be used for laboratory diagnosis propagated on much safer culture system of high biotechnology advances. © 2007 Science Publications.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.relationAmerican Journal of Biochemistry and Biotechnology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBoHV-5
dc.subjectBovine herpesvirus
dc.subjectCER cells
dc.subjectCRIB cells
dc.subjectMDBK cells
dc.subjectSerum-free media
dc.subjectAntibiotics
dc.subjectBiotechnology
dc.subjectCell growth
dc.subjectDiagnosis
dc.subjectProteins
dc.subjectBovine herpesvirus type 5 (BoHV-5)
dc.subjectViruses
dc.subjectAnimalia
dc.subjectBovinae
dc.subjectBovine herpesvirus 5
dc.subjectHerpesviridae
dc.titleBio-safety technology in production of bovine herpesvirus type 5 (BoHV-5) using an alternative serum-free medium
dc.typeOtro


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