In vitro metabolism effect on genotoxicity and antigenotoxicity of Agaricus blazei organics and aqueous extracts by the comet assay

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBellini, Marilanda Ferreira
dc.creatorTerezan, Ana Paula
dc.creatorRibeiro, Lúcia Regina
dc.creatorMantovani, Mário Sérgio
dc.date2014-05-27T11:21:57Z
dc.date2016-10-25T18:22:37Z
dc.date2014-05-27T11:21:57Z
dc.date2016-10-25T18:22:37Z
dc.date2006-09-01
dc.date.accessioned2017-04-06T01:20:12Z
dc.date.available2017-04-06T01:20:12Z
dc.identifierCytologia, v. 71, n. 3, p. 205-211, 2006.
dc.identifier0011-4545
dc.identifierhttp://hdl.handle.net/11449/69062
dc.identifierhttp://acervodigital.unesp.br/handle/11449/69062
dc.identifier10.1508/cytologia.71.205
dc.identifier2-s2.0-33750738093.pdf
dc.identifier2-s2.0-33750738093
dc.identifierhttp://dx.doi.org/10.1508/cytologia.71.205
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/890356
dc.descriptionThere is high interest in the natural products properties due to their use in popular medicine. Agaricus blazei Murrill ss. Heinem. (Ab) is native to Brazil and has been widely disseminated because its medicinal properties. In the present study, the genotoxic and antigenotoxic potential of Ab extracts were investigated using the comet assay. The cells utilized were the non drug-metabolizing line CHO-k1 (Chinese hamster ovary) and the drug-metabolizing line HTC (rat hepatoma). Cells were treated for 3 h in the absence of fetal bovain serum (FBS) with methanolic, hexanic and n-butanolic extracts at 50 μg/ml and 0.75% aqueous extract to test for genotoxicity. Antigenotoxic effects of extracts were determined in cells exposed to the DNA damage inducing agent ethyl methanesulfonate under simultaneous or simultaneous with 1 h pre-incubation conditions. The extracts did not show genotoxicity in HTC, while they were genotoxic in CHO-k1. No antigenotoxic effect was observed with any extract under any condition. These results demonstrate that the metabolism in presence or in absence has a direct influence on the genotoxicity of these extracts. © 2006 The Japan Mendel Society.
dc.languageeng
dc.relationCytologia
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAgaricus blazei
dc.subjectAntigenotoxicity
dc.subjectComet assay
dc.subjectGenotoxicity
dc.subjectMetabolism
dc.subjectCricetulus griseus
dc.titleIn vitro metabolism effect on genotoxicity and antigenotoxicity of Agaricus blazei organics and aqueous extracts by the comet assay
dc.typeOtro


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