Endothelial and neuronal nitric oxide synthase inhibitors influences angiotensin II pressor effect in central nervous system

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSaad, Wilson Abrão
dc.creatorGuarda, Ismael Francisco Motta Siqueira
dc.creatorCamargo, Luiz Antonio de Arreda
dc.creatorSaad, William Abrão
dc.creatorGuarda, Renata Saad
dc.creatorSantos, A. F. B. Talmir
dc.creatorSimões, Sylvio
dc.date2014-05-27T11:21:52Z
dc.date2016-10-25T18:22:10Z
dc.date2014-05-27T11:21:52Z
dc.date2016-10-25T18:22:10Z
dc.date2006-05-01
dc.date.accessioned2017-04-06T01:18:37Z
dc.date.available2017-04-06T01:18:37Z
dc.identifierInternational Journal of Pharmacology, v. 2, n. 3, p. 341-347, 2006.
dc.identifier1811-7775
dc.identifier1812-5700
dc.identifierhttp://hdl.handle.net/11449/68874
dc.identifierhttp://acervodigital.unesp.br/handle/11449/68874
dc.identifier10.3923/ijp.2006.341.347
dc.identifier2-s2.0-33745789046
dc.identifierhttp://dx.doi.org/10.3923/ijp.2006.341.347
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/890185
dc.descriptionThe present study investigated the central role of angiotensin II and nitric oxide on arterial blood pressure (MAP) in rats. Losartan and PD123349 AT 1 and AT 2 (selective no peptides antagonists angiotensin receptors), as well as FK 409 (a nitric oxide donor), N W-nitro-L-arginine methyl ester (L-NAME) a constituve nitric oxide synthase inhibitor endothelial (eNOSI) and 7-nitroindazol (7NI) a specific neuronal nitric oxide synthase inhibitor (nNOSI) were used. Holtzman strain, (Rattus norvergicus) weighting 200-250 g were anesthetized with zoletil 50 mg kg -1 (tiletamine chloridrate 125 mg and zolazepan chloridrate 125 mg) into quadriceps muscle anda stainless steel cannula was stereotaxically implanted into their Lateral Ventricle (LV). Controls were injected with a 0.5 μl volume of 0.15 M NaCl. Angiotensin II injected into LV increased MAP (19±3 vs. control 3±1 mm Hg), which is potentiated by prior injection of L-NAME in the same site 26±2 mm Hg. 7NI injected prior to ANG II into LV also potentiated the pressor effect of ANG II but with a higher intensity than L-NAME 32±3 mm Hg. FK 409 inhibited the pressor effect of ANG II (6±1 mm Hg). Losartan injected into LV before ANG II influences the pressor effect of ANG II (8±1 mm Hg). The PD 123319 decreased the pressor effects of ANG II (16±1 mm Hg). Losartan injected simultaneously with FK 409 blocked the pressor effect of ANG II (3±1 mm Hg). L-NAME produced an increase in the pressor effect of ANG II, may be due to local vasoconstriction and all at once by neuronal NOS inhibition but the main effect is of the 7-NIT an specific nNOS inhibitor. The AT 1 antagonist receptors improve basal nitric oxide (NO) production and release. These data suggest the involvement of constitutive and neuronal NOS in the control of arterial blood pressure induced by ANG II centrally, evolving AT 1 receptor-mediated vasoconstriction and AT 2 receptor-mediated vasodilatation. These results were confirmed by the experiment using FK 409. © 2006 Asian Network for Scientific Information.
dc.languageeng
dc.relationInternational Journal of Pharmacology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject7-nitroindazol
dc.subjectAngiotensin II receptor
dc.subjectArterial pressure
dc.subjectCNS
dc.subjectL-NAME
dc.subject4 ethyl 2 hydroxyimino 5 nitro 3 hexenamide
dc.subject7 nitroindazole
dc.subjectangiotensin 1 receptor
dc.subjectangiotensin 1 receptor antagonist
dc.subjectangiotensin 2 receptor
dc.subjectangiotensin 2 receptor antagonist
dc.subjectangiotensin II
dc.subjectendothelial nitric oxide synthase
dc.subjectlosartan
dc.subjectn(g) nitroarginine methyl ester
dc.subjectneuronal nitric oxide synthase
dc.subjectnitric oxide
dc.subjectnitric oxide synthase inhibitor
dc.subjectpd 123349
dc.subjectsodium chloride
dc.subjecttelazol
dc.subjectunclassified drug
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectblood pressure regulation
dc.subjectcentral nervous system
dc.subjectcontrolled study
dc.subjectdrug specificity
dc.subjectenzyme inhibition
dc.subjectlateral brain ventricle
dc.subjectmean arterial pressure
dc.subjectnonhuman
dc.subjectpressor response
dc.subjectpressure measurement
dc.subjectquadriceps femoris muscle
dc.subjectrat
dc.subjectvasoconstriction
dc.subjectvasodilatation
dc.titleEndothelial and neuronal nitric oxide synthase inhibitors influences angiotensin II pressor effect in central nervous system
dc.typeOtro


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