The number of podocyte and slit diaphragm is decreased in experimental diabetic nephropathy

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorLerco, Mauro Masson
dc.creatorMacedo, Célia Sperandeo
dc.creatorSilva, Reinaldo José
dc.creatorPinheiro, Daniela de Oliveira
dc.creatorSpadella, César Tadeu
dc.date2014-05-27T11:21:49Z
dc.date2016-10-25T18:21:59Z
dc.date2014-05-27T11:21:49Z
dc.date2016-10-25T18:21:59Z
dc.date2006-03-01
dc.date.accessioned2017-04-06T01:17:57Z
dc.date.available2017-04-06T01:17:57Z
dc.identifierActa Cirurgica Brasileira, v. 21, n. 2, p. 87-91, 2006.
dc.identifier0102-8650
dc.identifier1678-2674
dc.identifierhttp://hdl.handle.net/11449/68794
dc.identifierhttp://acervodigital.unesp.br/handle/11449/68794
dc.identifier10.1590/S0102-86502006000200006
dc.identifierS0102-86502006000200006
dc.identifier2-s2.0-33645788562.pdf
dc.identifier2-s2.0-33645788562
dc.identifierhttp://dx.doi.org/10.1590/S0102-86502006000200006
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/890108
dc.descriptionPurpose: To determine the number of podocyte, slit diaphragms, slit diaphragm extensions and GBM thickness in diabetic nephropathy. Methods: Sixty Rattus Wistarof both sexes weighing 200-300g were divided in two experimental groups: normal group 10 animals, and alloxan diabetic rats - 50 animals. Alloxan was administered in a single IV dose of 42mg/kg body weight. Body weight, water and food intake, diuresis, and blood and urine glucose were determined in both groups before alloxan injection and two weeks, six and twelve months after alloxan injection. Proteinuria was measured at 12 months in both groups. After 12 months animals were sacrificed, and the right kidney processed for electron microscopy. Results: Clear clinical and laboratory signs of severe diabetes were seen, in all alloxan-diabetic rats at all follow-up times. Glomerular basement membrane (GBM) thickening, podocyte number, and slit diaphragm number and extension were determined. GBM of all diabetic rats was significantly thicker (median=0.29μm; semi-interquartile range=0.065μm) than in the normal rats (0.23μm; 0.035μm). Diabetic rat podocyte number (8; 1), slit diaphragm number (4; 1), and slit diaphragm extension (0.021μm; 0.00435μm) were significantly lower than in normal rats (11; 1) and (7; 1.5), and (0.031μm; 0.0058μm). Diabetic rat proteinuria (0.060mg/24h; 0.037mg/24h) was higher than in normal rats (0.00185mg/24h; 0.00055mg/24h). Conclusion: Experimental diabetes is associated with significant (p<0.05) changes in podocyte foot process, slit number, slit diaphragm extension, and GBM thickness.
dc.languageeng
dc.relationActa Cirúrgica Brasileira
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDiabetes Mellitus
dc.subjectElectron
dc.subjectGlomerulonephritis
dc.subjectMembranous
dc.subjectMicroscopy
dc.subjectalloxan
dc.subjectglucose
dc.subjectalloxan diabetes mellitus
dc.subjectanimal euthanasia
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectbody weight
dc.subjectcell count
dc.subjectcell membrane
dc.subjectcontrolled study
dc.subjectdiabetic nephropathy
dc.subjectelectron microscopy
dc.subjectfemale
dc.subjectfluid intake
dc.subjectfood intake
dc.subjectglomerulus basement membrane
dc.subjectglucose blood level
dc.subjectglucose urine level
dc.subjectkidney
dc.subjectlaboratory test
dc.subjectmale
dc.subjectnonhuman
dc.subjectpodocyte
dc.subjectproteinuria
dc.subjectrat
dc.subjectspecies comparison
dc.subjectAnimals
dc.subjectCell Count
dc.subjectDiabetes Mellitus, Experimental
dc.subjectDiabetic Nephropathies
dc.subjectDisease Progression
dc.subjectFemale
dc.subjectGlomerular Basement Membrane
dc.subjectMale
dc.subjectMicroscopy, Electron
dc.subjectPodocytes
dc.subjectRats
dc.subjectRats, Wistar
dc.titleThe number of podocyte and slit diaphragm is decreased in experimental diabetic nephropathy
dc.typeOtro


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