ITS1 intra-individual variability of Ascaris isolates from Brazil

dc.creatorLeles, Daniela
dc.creatorAraújo, Adauto
dc.creatorVicente, Ana Carolina Paulo
dc.creatorIñiguez, Alena Mayo
dc.date2018-12-04T10:23:10Z
dc.date2018-12-04T10:23:10Z
dc.date2010
dc.date.accessioned2023-09-27T00:15:21Z
dc.date.available2023-09-27T00:15:21Z
dc.identifierLELES, Daniela; et al. ITS1 intra-individual variability of Ascaris isolates from Brazil. Parasitology International, v.59, p.93–96, 2010.
dc.identifier1383-5769
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/30375
dc.identifier10.1016/j.parint.2009.10.001
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8899008
dc.descriptionThe zoonotic potential of Ascaris infecting pigs has stimulated studies of molecular epidemiology with internal transcribed spacer 1 (ITS1) as the target. The aim of this study was to determine the value of Ascaris ITS1 as a molecular marker through assessing the intra-individual genetic diversity of Ascaris isolates from two geographical areas of Brazil. DNA was extracted from single isolated eggs, ITS1 PCR was performed, and the PCR products were cloned and sequenced. Clone analysis showed high ITS1 intra-individual variability revealed by 2–4 ITS1 genotypes/haplotypes per sample (egg). Two genotypes, G1 and G6, and 13 new haplotypes were detected and characterized. The most prevalent in humans, G1 and/or the Brazilian G6, were detected in all samples. Except for genotype G1, no relationship was observed between Brazilian ITS1 genotypes/haplotypes and those previously described in China, Bangladesh, Japan, United Kingdom, Australia, and Denmark, with respect to geographic origin or host affiliation. However, an association between the two geographically separated Brazilian ITS1 isolates was observed. The ITS1 intra-individual variability revealed in this study indicated that the use of this genetic region to discriminate human and pig Ascaris genotypes should be reconsidered.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectAscaris
dc.subjectNematódeos
dc.subjectDiagnóstico molecular
dc.subjectCaracterização genética
dc.subjectBrasil
dc.subjectAscaris
dc.subjectBrazilian isolates
dc.subjectITS intra-individual variability
dc.subjectNematodes
dc.subjectGenetic characterization
dc.subjectMolecular diagnosis
dc.titleITS1 intra-individual variability of Ascaris isolates from Brazil
dc.typeArticle


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