The Rhipicephalus sanguineus (Latreille) complex (Acari:Ixodidae) is composed of species with intra- and interspecific morphological variation that make their diagnosis difficult. In the present study, male specimens of the R. sanguineus complex were collected from dogs in six districts of three regions of Brazil and submitted to molecular and scanning electron microscopy (SEM) analyses. Analysis of COX1 gene, 12S rDNA, and D-loop rDNA shows that ticks classified as R. sanguineus form two different clades. Morphological comparisons using SEM found adult males to exhibit morphological differences in Haller's organ, festoons, and adanal, spiracular, and genital plates, with the last having potential usefulness in distinguishing male specimens of the complex.2030-01-01