Diversity and molecular characterization of novel hemoplasmas infecting wild rodents from different Brazilian biomes

dc.creatorGonçalves, Luiz Ricardo
dc.creatorRoque, André Luiz Rodrigues
dc.creatorMatos, Carlos Antonio
dc.creatorFernandes, Simone de Jesus
dc.creatorOlmos, Isabella Delamain Fernandez
dc.creatorMachado, Rosangela Zacarias
dc.creatorAndré, Marcos Rogério
dc.date2016-03-17T16:21:15Z
dc.date2016-03-17T16:21:15Z
dc.date2015
dc.date.accessioned2023-09-27T00:12:15Z
dc.date.available2023-09-27T00:12:15Z
dc.identifierGONÇALVES, Luiz Ricardo; et al. Diversity and molecular characterization of novel hemoplasmas infecting wild rodents from different Brazilian biomes. Comparative Immunology, Microbiology and Infectious Diseases, v.43, p.50-56, Dec. 2015.
dc.identifier0147-9571
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/13201
dc.identifier10.1016/j.cimid.2015.10.006
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898488
dc.descriptionAlthough hemoplasma infection in domestic animals has been well documented, little is known about the prevalence and genetic diversity of these bacteria in wild rodents. The present work aimed to investigate the occurrence of hemotrophic mycoplasmas in wild rodents from five Brazilian biomes, assessing the 16S rRNA phylogenetic position of hemoplasma species by molecular approach. Spleen tissues were obtained from 500 rodents, comprising 52 different rodent species trapped between 2000 and 2011. DNA samples were submitted to previously described PCR protocols for amplifying Mycoplasma spp. based on 16S rRNA, followed by sequencing and phylogenetic inferences. Among 457 rodent spleen samples showing absence of inhibitors, 100 (21.9%) were PCR positive to Mycoplasma spp. The occurrence of hemotropic mycoplasmas among all sampled rodents was demonstrated in all five biomes and ranged from 9.3% (7/75) to 26.2% (38/145). The Blastn analysis showed that amplified sequences had a percentage of identity ranging from 86 to 99% with other murine hemoplasmas. The ML phylogenetic analysis of 16S rRNA gene of 24 positive randomly selected samples showed the presence of ten distinct groups, all clustering within the Mycoplasma haemofelis. The phylogenetic assessment suggests the circulation of novel hemoplasma species in rodents from different biomes in Brazil.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectHemotropic mycoplasmas
dc.subjectWild rodents
dc.subjectMolecular characterization
dc.subjectBrazil
dc.subjectRoedores
dc.subjectBrasil
dc.titleDiversity and molecular characterization of novel hemoplasmas infecting wild rodents from different Brazilian biomes
dc.typeArticle


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