Trachycephalus cunauaru occurs in Brazil, Bolivia, Peru and probably Ecuador and Venezuela (Gordo et al. 2013; Frost, 2016). The Negro and Amazonas rivers possibly represent geographical barriers to the species in the central Amazon region by isolating the same congener Trachycephalus resinifictrix, to which the river Xingu apparently also represents a geographic boundary to the species to southeastern Amazon (Gordo et al. 2013). In Brazil the species is recorded for the central region of the state of Amazonas, south and southwest of the state of Pará, east of Acre and north of Mato Grosso (Gordo et al. 2013).