| dc.creator | Cardoso, Liana Monteiro da Fonseca | |
| dc.creator | Pinto, Marcelo Alves | |
| dc.creator | Pons, Andrea Henriques | |
| dc.creator | Alves, Luiz Anastácio | |
| dc.date | 2018-10-25T14:28:21Z | |
| dc.date | 2018-10-25T14:28:21Z | |
| dc.date | 2018 | |
| dc.date.accessioned | 2023-09-27T00:05:26Z | |
| dc.date.available | 2023-09-27T00:05:26Z | |
| dc.identifier | CARDOSO, Liana Monteiro da Fonseca; et al. Cryopreservation of rat hepatocytes with disaccharides for cell therapy. Cryobiology, v.78, p.15-21, Aug. 2018. | |
| dc.identifier | 0011-2240 | |
| dc.identifier | https://www.arca.fiocruz.br/handle/icict/29720 | |
| dc.identifier | 10.1016/j.cryobiol.2017.07.010 | |
| dc.identifier | 1025-496X | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8897326 | |
| dc.description | Cryopreservation of hepatocytes is a crucial step in the implementation of cell therapy for treating
certain liver diseases. In the present study we investigated the effect of the some disaccharides on the
cryopreservation of rat hepatocytes. Liver cells were frozen in media in the presence or absence of low
concentrations of Me2SO (5% Me2SO) supplemented with varying concentrations of disaccharides (sucrose,
glucose and trehalose). After 7 days of cryopreservation, the hepatocytes were thawed and
viability was measure by exclusion of trypan blue and by the MTT technique, as well as by determining
albumin production. Among the investigated disaccharides and concentrations, 0.2 M trehalose showed
the best overall outcome. Compared to the use of Me2SO alone, significant improvement in post-thaw
cell viability was observed. The new solution may reduce Me2SO side effects on patients and improve
the viability and quality of cryopreserved hepatocytes. | |
| dc.description | 2030-01-01 | |
| dc.format | application/pdf | |
| dc.language | eng | |
| dc.publisher | European Centre for Disease Prevention and Control | |
| dc.rights | restricted access | |
| dc.subject | Hepatócito | |
| dc.subject | Criopreservação | |
| dc.subject | Dissacarídeos | |
| dc.subject | Hepatocyte | |
| dc.subject | Cryopreservation | |
| dc.subject | Cell therapy | |
| dc.subject | Disaccharides | |
| dc.subject | Thehalose | |
| dc.subject | Terapia Baseada em Transplante de Células e Tecidos | |
| dc.title | Cryopreservation of rat hepatocytes with disaccharides for cell therapy | |
| dc.type | Article | |
